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Recurrent Evolution of Melanism in South American Felids

Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorph...

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Autores principales: Schneider, Alexsandra, Henegar, Corneliu, Day, Kenneth, Absher, Devin, Napolitano, Constanza, Silveira, Leandro, David, Victor A., O’Brien, Stephen J., Menotti-Raymond, Marilyn, Barsh, Gregory S., Eizirik, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335015/
https://www.ncbi.nlm.nih.gov/pubmed/25695801
http://dx.doi.org/10.1371/journal.pgen.1004892
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author Schneider, Alexsandra
Henegar, Corneliu
Day, Kenneth
Absher, Devin
Napolitano, Constanza
Silveira, Leandro
David, Victor A.
O’Brien, Stephen J.
Menotti-Raymond, Marilyn
Barsh, Gregory S.
Eizirik, Eduardo
author_facet Schneider, Alexsandra
Henegar, Corneliu
Day, Kenneth
Absher, Devin
Napolitano, Constanza
Silveira, Leandro
David, Victor A.
O’Brien, Stephen J.
Menotti-Raymond, Marilyn
Barsh, Gregory S.
Eizirik, Eduardo
author_sort Schneider, Alexsandra
collection PubMed
description Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy’s cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from 54 individuals (13–21 per species), achieving enrichment of ~500–2500-fold and ~150x coverage. Our analysis points to unique evolutionary histories for each of the three species, with a strong selective sweep in the pampas cat, a distinctive but short melanism-specific haplotype in the Geoffroy’s cat, and reduced nucleotide diversity for both ancestral and melanism-bearing chromosomes in the kodkod. These results reveal an important role for natural selection in a trait of longstanding interest to ecologists, geneticists, and the lay community, and provide a platform for comparative studies of morphological variation in other natural populations.
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spelling pubmed-43350152015-02-24 Recurrent Evolution of Melanism in South American Felids Schneider, Alexsandra Henegar, Corneliu Day, Kenneth Absher, Devin Napolitano, Constanza Silveira, Leandro David, Victor A. O’Brien, Stephen J. Menotti-Raymond, Marilyn Barsh, Gregory S. Eizirik, Eduardo PLoS Genet Research Article Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy’s cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from 54 individuals (13–21 per species), achieving enrichment of ~500–2500-fold and ~150x coverage. Our analysis points to unique evolutionary histories for each of the three species, with a strong selective sweep in the pampas cat, a distinctive but short melanism-specific haplotype in the Geoffroy’s cat, and reduced nucleotide diversity for both ancestral and melanism-bearing chromosomes in the kodkod. These results reveal an important role for natural selection in a trait of longstanding interest to ecologists, geneticists, and the lay community, and provide a platform for comparative studies of morphological variation in other natural populations. Public Library of Science 2015-02-19 /pmc/articles/PMC4335015/ /pubmed/25695801 http://dx.doi.org/10.1371/journal.pgen.1004892 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Schneider, Alexsandra
Henegar, Corneliu
Day, Kenneth
Absher, Devin
Napolitano, Constanza
Silveira, Leandro
David, Victor A.
O’Brien, Stephen J.
Menotti-Raymond, Marilyn
Barsh, Gregory S.
Eizirik, Eduardo
Recurrent Evolution of Melanism in South American Felids
title Recurrent Evolution of Melanism in South American Felids
title_full Recurrent Evolution of Melanism in South American Felids
title_fullStr Recurrent Evolution of Melanism in South American Felids
title_full_unstemmed Recurrent Evolution of Melanism in South American Felids
title_short Recurrent Evolution of Melanism in South American Felids
title_sort recurrent evolution of melanism in south american felids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335015/
https://www.ncbi.nlm.nih.gov/pubmed/25695801
http://dx.doi.org/10.1371/journal.pgen.1004892
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