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The Ino80 complex prevents invasion of euchromatin into silent chromatin
Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncod...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335291/ https://www.ncbi.nlm.nih.gov/pubmed/25691465 http://dx.doi.org/10.1101/gad.256255.114 |
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author | Xue, Yong Van, Christopher Pradhan, Suman K. Su, Trent Gehrke, Jason Kuryan, Benjamin G. Kitada, Tasuku Vashisht, Ajay Tran, Nancy Wohlschlegel, James Peterson, Craig L. Kurdistani, Siavash K. Carey, Michael F. |
author_facet | Xue, Yong Van, Christopher Pradhan, Suman K. Su, Trent Gehrke, Jason Kuryan, Benjamin G. Kitada, Tasuku Vashisht, Ajay Tran, Nancy Wohlschlegel, James Peterson, Craig L. Kurdistani, Siavash K. Carey, Michael F. |
author_sort | Xue, Yong |
collection | PubMed |
description | Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin. |
format | Online Article Text |
id | pubmed-4335291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43352912015-08-15 The Ino80 complex prevents invasion of euchromatin into silent chromatin Xue, Yong Van, Christopher Pradhan, Suman K. Su, Trent Gehrke, Jason Kuryan, Benjamin G. Kitada, Tasuku Vashisht, Ajay Tran, Nancy Wohlschlegel, James Peterson, Craig L. Kurdistani, Siavash K. Carey, Michael F. Genes Dev Research Communication Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin. Cold Spring Harbor Laboratory Press 2015-02-15 /pmc/articles/PMC4335291/ /pubmed/25691465 http://dx.doi.org/10.1101/gad.256255.114 Text en © 2015 Xue et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Communication Xue, Yong Van, Christopher Pradhan, Suman K. Su, Trent Gehrke, Jason Kuryan, Benjamin G. Kitada, Tasuku Vashisht, Ajay Tran, Nancy Wohlschlegel, James Peterson, Craig L. Kurdistani, Siavash K. Carey, Michael F. The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title | The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title_full | The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title_fullStr | The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title_full_unstemmed | The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title_short | The Ino80 complex prevents invasion of euchromatin into silent chromatin |
title_sort | ino80 complex prevents invasion of euchromatin into silent chromatin |
topic | Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335291/ https://www.ncbi.nlm.nih.gov/pubmed/25691465 http://dx.doi.org/10.1101/gad.256255.114 |
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