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The Ino80 complex prevents invasion of euchromatin into silent chromatin

Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncod...

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Autores principales: Xue, Yong, Van, Christopher, Pradhan, Suman K., Su, Trent, Gehrke, Jason, Kuryan, Benjamin G., Kitada, Tasuku, Vashisht, Ajay, Tran, Nancy, Wohlschlegel, James, Peterson, Craig L., Kurdistani, Siavash K., Carey, Michael F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335291/
https://www.ncbi.nlm.nih.gov/pubmed/25691465
http://dx.doi.org/10.1101/gad.256255.114
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author Xue, Yong
Van, Christopher
Pradhan, Suman K.
Su, Trent
Gehrke, Jason
Kuryan, Benjamin G.
Kitada, Tasuku
Vashisht, Ajay
Tran, Nancy
Wohlschlegel, James
Peterson, Craig L.
Kurdistani, Siavash K.
Carey, Michael F.
author_facet Xue, Yong
Van, Christopher
Pradhan, Suman K.
Su, Trent
Gehrke, Jason
Kuryan, Benjamin G.
Kitada, Tasuku
Vashisht, Ajay
Tran, Nancy
Wohlschlegel, James
Peterson, Craig L.
Kurdistani, Siavash K.
Carey, Michael F.
author_sort Xue, Yong
collection PubMed
description Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin.
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spelling pubmed-43352912015-08-15 The Ino80 complex prevents invasion of euchromatin into silent chromatin Xue, Yong Van, Christopher Pradhan, Suman K. Su, Trent Gehrke, Jason Kuryan, Benjamin G. Kitada, Tasuku Vashisht, Ajay Tran, Nancy Wohlschlegel, James Peterson, Craig L. Kurdistani, Siavash K. Carey, Michael F. Genes Dev Research Communication Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin. Cold Spring Harbor Laboratory Press 2015-02-15 /pmc/articles/PMC4335291/ /pubmed/25691465 http://dx.doi.org/10.1101/gad.256255.114 Text en © 2015 Xue et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Xue, Yong
Van, Christopher
Pradhan, Suman K.
Su, Trent
Gehrke, Jason
Kuryan, Benjamin G.
Kitada, Tasuku
Vashisht, Ajay
Tran, Nancy
Wohlschlegel, James
Peterson, Craig L.
Kurdistani, Siavash K.
Carey, Michael F.
The Ino80 complex prevents invasion of euchromatin into silent chromatin
title The Ino80 complex prevents invasion of euchromatin into silent chromatin
title_full The Ino80 complex prevents invasion of euchromatin into silent chromatin
title_fullStr The Ino80 complex prevents invasion of euchromatin into silent chromatin
title_full_unstemmed The Ino80 complex prevents invasion of euchromatin into silent chromatin
title_short The Ino80 complex prevents invasion of euchromatin into silent chromatin
title_sort ino80 complex prevents invasion of euchromatin into silent chromatin
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335291/
https://www.ncbi.nlm.nih.gov/pubmed/25691465
http://dx.doi.org/10.1101/gad.256255.114
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