Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China

A duck tembusu virus (DTMUV) was isolated from the brain of a Cherry Valley duckling that showed neurological signs by using a specific-pathogen-free chicken embryo. The isolate was named GX2013G (GenBank accession no. KM275941). The strain GX2013G was identified with reverse transcription-PCR (RT-P...

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Autores principales: Zeng, Tingting, Xie, Zhixun, Xie, Liji, Deng, Xianwen, Xie, Zhiqin, Huang, Li, Luo, Sisi, Huang, Jiaoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335318/
https://www.ncbi.nlm.nih.gov/pubmed/25700393
http://dx.doi.org/10.1128/genomeA.00007-15
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author Zeng, Tingting
Xie, Zhixun
Xie, Liji
Deng, Xianwen
Xie, Zhiqin
Huang, Li
Luo, Sisi
Huang, Jiaoling
author_facet Zeng, Tingting
Xie, Zhixun
Xie, Liji
Deng, Xianwen
Xie, Zhiqin
Huang, Li
Luo, Sisi
Huang, Jiaoling
author_sort Zeng, Tingting
collection PubMed
description A duck tembusu virus (DTMUV) was isolated from the brain of a Cherry Valley duckling that showed neurological signs by using a specific-pathogen-free chicken embryo. The isolate was named GX2013G (GenBank accession no. KM275941). The strain GX2013G was identified with reverse transcription-PCR (RT-PCR), and the amplicon was sequenced. The genome that was obtained is 10,990 nucleotides in length and contains a single open reading frame encoding a putative polyprotein of 3,425 amino acids. This study will advance the understanding of the epidemiology and molecular characteristics of tembusu virus (TMUV) in Guangxi and further studies of the mechanisms of virus replication and pathogenesis.
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spelling pubmed-43353182015-02-20 Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China Zeng, Tingting Xie, Zhixun Xie, Liji Deng, Xianwen Xie, Zhiqin Huang, Li Luo, Sisi Huang, Jiaoling Genome Announc Viruses A duck tembusu virus (DTMUV) was isolated from the brain of a Cherry Valley duckling that showed neurological signs by using a specific-pathogen-free chicken embryo. The isolate was named GX2013G (GenBank accession no. KM275941). The strain GX2013G was identified with reverse transcription-PCR (RT-PCR), and the amplicon was sequenced. The genome that was obtained is 10,990 nucleotides in length and contains a single open reading frame encoding a putative polyprotein of 3,425 amino acids. This study will advance the understanding of the epidemiology and molecular characteristics of tembusu virus (TMUV) in Guangxi and further studies of the mechanisms of virus replication and pathogenesis. American Society for Microbiology 2015-02-19 /pmc/articles/PMC4335318/ /pubmed/25700393 http://dx.doi.org/10.1128/genomeA.00007-15 Text en Copyright © 2015 Zeng et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Viruses
Zeng, Tingting
Xie, Zhixun
Xie, Liji
Deng, Xianwen
Xie, Zhiqin
Huang, Li
Luo, Sisi
Huang, Jiaoling
Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title_full Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title_fullStr Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title_full_unstemmed Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title_short Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China
title_sort identification and whole-genome sequence analysis of tembusu virus gx2013g, isolated from a cherry valley duckling in southern china
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335318/
https://www.ncbi.nlm.nih.gov/pubmed/25700393
http://dx.doi.org/10.1128/genomeA.00007-15
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