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Intestinal Tumor in a Dish
Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy duri...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335404/ https://www.ncbi.nlm.nih.gov/pubmed/25705626 http://dx.doi.org/10.3389/fmed.2014.00014 |
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author | Ohta, Yuki Sato, Toshiro |
author_facet | Ohta, Yuki Sato, Toshiro |
author_sort | Ohta, Yuki |
collection | PubMed |
description | Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy during drug development. Recent advances in genetic sequencing has allowed us to improve the prediction of drug response through genetic stratification of patients into smaller populations, yet the complexity of the cancer genome still often confounds accuracy of drug response prediction. Ultimately, we may need to replicate patient’s own tumor in a dish in order to test drug responses so that the optimal treatment can be identified. We recently developed highly efficient and tractable organoid culture system for intestinal stem cells, in which single stem cells form 3D structures recapitulating original tissue architecture. This technology has also been applied to colorectal tumors and enables us to monitor the growth and response of the patient’s own tumors. In this review, we provide an overview focusing on CRC organoid culture and its perspective for clinical applications. |
format | Online Article Text |
id | pubmed-4335404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43354042015-02-20 Intestinal Tumor in a Dish Ohta, Yuki Sato, Toshiro Front Med (Lausanne) Medicine Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy during drug development. Recent advances in genetic sequencing has allowed us to improve the prediction of drug response through genetic stratification of patients into smaller populations, yet the complexity of the cancer genome still often confounds accuracy of drug response prediction. Ultimately, we may need to replicate patient’s own tumor in a dish in order to test drug responses so that the optimal treatment can be identified. We recently developed highly efficient and tractable organoid culture system for intestinal stem cells, in which single stem cells form 3D structures recapitulating original tissue architecture. This technology has also been applied to colorectal tumors and enables us to monitor the growth and response of the patient’s own tumors. In this review, we provide an overview focusing on CRC organoid culture and its perspective for clinical applications. Frontiers Media S.A. 2014-05-30 /pmc/articles/PMC4335404/ /pubmed/25705626 http://dx.doi.org/10.3389/fmed.2014.00014 Text en Copyright © 2014 Ohta and Sato. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Ohta, Yuki Sato, Toshiro Intestinal Tumor in a Dish |
title | Intestinal Tumor in a Dish |
title_full | Intestinal Tumor in a Dish |
title_fullStr | Intestinal Tumor in a Dish |
title_full_unstemmed | Intestinal Tumor in a Dish |
title_short | Intestinal Tumor in a Dish |
title_sort | intestinal tumor in a dish |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335404/ https://www.ncbi.nlm.nih.gov/pubmed/25705626 http://dx.doi.org/10.3389/fmed.2014.00014 |
work_keys_str_mv | AT ohtayuki intestinaltumorinadish AT satotoshiro intestinaltumorinadish |