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Intestinal Tumor in a Dish

Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy duri...

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Detalles Bibliográficos
Autores principales: Ohta, Yuki, Sato, Toshiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335404/
https://www.ncbi.nlm.nih.gov/pubmed/25705626
http://dx.doi.org/10.3389/fmed.2014.00014
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author Ohta, Yuki
Sato, Toshiro
author_facet Ohta, Yuki
Sato, Toshiro
author_sort Ohta, Yuki
collection PubMed
description Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy during drug development. Recent advances in genetic sequencing has allowed us to improve the prediction of drug response through genetic stratification of patients into smaller populations, yet the complexity of the cancer genome still often confounds accuracy of drug response prediction. Ultimately, we may need to replicate patient’s own tumor in a dish in order to test drug responses so that the optimal treatment can be identified. We recently developed highly efficient and tractable organoid culture system for intestinal stem cells, in which single stem cells form 3D structures recapitulating original tissue architecture. This technology has also been applied to colorectal tumors and enables us to monitor the growth and response of the patient’s own tumors. In this review, we provide an overview focusing on CRC organoid culture and its perspective for clinical applications.
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spelling pubmed-43354042015-02-20 Intestinal Tumor in a Dish Ohta, Yuki Sato, Toshiro Front Med (Lausanne) Medicine Predicting the response of colorectal cancer (CRC) tumors to novel chemotherapeutic agents is significantly complicated by their underlying genetic and epigenetic diversity. Large-scale clinical trials involving thousands of patients are often necessary in order to accurately determine efficacy during drug development. Recent advances in genetic sequencing has allowed us to improve the prediction of drug response through genetic stratification of patients into smaller populations, yet the complexity of the cancer genome still often confounds accuracy of drug response prediction. Ultimately, we may need to replicate patient’s own tumor in a dish in order to test drug responses so that the optimal treatment can be identified. We recently developed highly efficient and tractable organoid culture system for intestinal stem cells, in which single stem cells form 3D structures recapitulating original tissue architecture. This technology has also been applied to colorectal tumors and enables us to monitor the growth and response of the patient’s own tumors. In this review, we provide an overview focusing on CRC organoid culture and its perspective for clinical applications. Frontiers Media S.A. 2014-05-30 /pmc/articles/PMC4335404/ /pubmed/25705626 http://dx.doi.org/10.3389/fmed.2014.00014 Text en Copyright © 2014 Ohta and Sato. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Ohta, Yuki
Sato, Toshiro
Intestinal Tumor in a Dish
title Intestinal Tumor in a Dish
title_full Intestinal Tumor in a Dish
title_fullStr Intestinal Tumor in a Dish
title_full_unstemmed Intestinal Tumor in a Dish
title_short Intestinal Tumor in a Dish
title_sort intestinal tumor in a dish
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335404/
https://www.ncbi.nlm.nih.gov/pubmed/25705626
http://dx.doi.org/10.3389/fmed.2014.00014
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