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Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study
BACKGROUND: Peridural fibrosis (PF) is a normal complication after lumbar surgery. It is a challenge for both surgeons and patients. Rapamycin (RPM), a novel antibiotic with anti-proliferative and immunosuppressive properties, has been shown to be effective in preventing uncontrolled scar proliferat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335565/ https://www.ncbi.nlm.nih.gov/pubmed/25677111 http://dx.doi.org/10.12659/MSM.893165 |
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author | Luo, Like Zhang, Chifei Zhao, Jinmin Wei, Qingjun Li, Xiaofeng |
author_facet | Luo, Like Zhang, Chifei Zhao, Jinmin Wei, Qingjun Li, Xiaofeng |
author_sort | Luo, Like |
collection | PubMed |
description | BACKGROUND: Peridural fibrosis (PF) is a normal complication after lumbar surgery. It is a challenge for both surgeons and patients. Rapamycin (RPM), a novel antibiotic with anti-proliferative and immunosuppressive properties, has been shown to be effective in preventing uncontrolled scar proliferation diseases. The object of the present research was to investigate the effects of RPM on inhibiting PF in vitro and in vivo. MATERIAL/METHODS: In vitro, the fibroblasts collected and isolated from the rat tail skin were cultured with/without RPM and cell counting was performed. In vivo, the double-blinded study was conducted in 60 healthy Wistar rats divided randomly into 3 groups: 1) RPM treatment group; 2) Vehicle treatment group; 3) Control group. Rats underwent a L1-L2 level laminectomy with a satisfactory anesthetization. Four weeks post-operatively, the Rydell score, histological analysis, hydroxyproline content, vimentin expressional level, and inflammatory cytokines expressional levels were assessed. RESULTS: In vitro, RPM showed ability to prevent fibroblast proliferation. In vivo, the laminectomy was well tolerated by all rats, which were killed 4 weeks post-operatively. The Rydell score, histological evaluation, hydroxyproline content, vimentin expression level, and inflammatory activity showed the positive effect of RPM in preventing peridural adhesion, inhibiting fibrotic formation and collagen synthesis, and down-regulating inflammation. CONCLUSIONS: In the present primary study, RPM showed good efficacy in preventing the proliferation of fibroblasts. RPM can prevent rat peridural adhesion through inhibiting collagen synthesis, fibroblasts proliferation, and inflammatory activity. |
format | Online Article Text |
id | pubmed-4335565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43355652015-03-13 Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study Luo, Like Zhang, Chifei Zhao, Jinmin Wei, Qingjun Li, Xiaofeng Med Sci Monit Animal Study BACKGROUND: Peridural fibrosis (PF) is a normal complication after lumbar surgery. It is a challenge for both surgeons and patients. Rapamycin (RPM), a novel antibiotic with anti-proliferative and immunosuppressive properties, has been shown to be effective in preventing uncontrolled scar proliferation diseases. The object of the present research was to investigate the effects of RPM on inhibiting PF in vitro and in vivo. MATERIAL/METHODS: In vitro, the fibroblasts collected and isolated from the rat tail skin were cultured with/without RPM and cell counting was performed. In vivo, the double-blinded study was conducted in 60 healthy Wistar rats divided randomly into 3 groups: 1) RPM treatment group; 2) Vehicle treatment group; 3) Control group. Rats underwent a L1-L2 level laminectomy with a satisfactory anesthetization. Four weeks post-operatively, the Rydell score, histological analysis, hydroxyproline content, vimentin expressional level, and inflammatory cytokines expressional levels were assessed. RESULTS: In vitro, RPM showed ability to prevent fibroblast proliferation. In vivo, the laminectomy was well tolerated by all rats, which were killed 4 weeks post-operatively. The Rydell score, histological evaluation, hydroxyproline content, vimentin expression level, and inflammatory activity showed the positive effect of RPM in preventing peridural adhesion, inhibiting fibrotic formation and collagen synthesis, and down-regulating inflammation. CONCLUSIONS: In the present primary study, RPM showed good efficacy in preventing the proliferation of fibroblasts. RPM can prevent rat peridural adhesion through inhibiting collagen synthesis, fibroblasts proliferation, and inflammatory activity. International Scientific Literature, Inc. 2015-02-13 /pmc/articles/PMC4335565/ /pubmed/25677111 http://dx.doi.org/10.12659/MSM.893165 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Animal Study Luo, Like Zhang, Chifei Zhao, Jinmin Wei, Qingjun Li, Xiaofeng Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title | Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title_full | Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title_fullStr | Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title_full_unstemmed | Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title_short | Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study |
title_sort | effects of rapamycin on reduction of peridural fibrosis: an experimental study |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335565/ https://www.ncbi.nlm.nih.gov/pubmed/25677111 http://dx.doi.org/10.12659/MSM.893165 |
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