DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis

We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as se...

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Detalles Bibliográficos
Autores principales: Koussa, Mounir A, Halvorsen, Ken, Ward, Andrew, Wong, Wesley P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336243/
https://www.ncbi.nlm.nih.gov/pubmed/25486062
http://dx.doi.org/10.1038/nmeth.3209
Descripción
Sumario:We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states.

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