DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis

We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as se...

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Detalles Bibliográficos
Autores principales: Koussa, Mounir A, Halvorsen, Ken, Ward, Andrew, Wong, Wesley P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336243/
https://www.ncbi.nlm.nih.gov/pubmed/25486062
http://dx.doi.org/10.1038/nmeth.3209
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author Koussa, Mounir A
Halvorsen, Ken
Ward, Andrew
Wong, Wesley P
author_facet Koussa, Mounir A
Halvorsen, Ken
Ward, Andrew
Wong, Wesley P
author_sort Koussa, Mounir A
collection PubMed
description We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states.
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spelling pubmed-43362432015-08-01 DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis Koussa, Mounir A Halvorsen, Ken Ward, Andrew Wong, Wesley P Nat Methods Article We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states. 2014-12-08 2015-02 /pmc/articles/PMC4336243/ /pubmed/25486062 http://dx.doi.org/10.1038/nmeth.3209 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Koussa, Mounir A
Halvorsen, Ken
Ward, Andrew
Wong, Wesley P
DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title_full DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title_fullStr DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title_full_unstemmed DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title_short DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
title_sort dna nanoswitches: a quantitative platform for gel-based biomolecular interaction analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336243/
https://www.ncbi.nlm.nih.gov/pubmed/25486062
http://dx.doi.org/10.1038/nmeth.3209
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