Cargando…

Effects of Macromolecular Crowding on the Structure of a Protein Complex: A Small-Angle Scattering Study of Superoxide Dismutase

Macromolecular crowding can alter the structure and function of biological macromolecules. We used small-angle scattering to measure the effects of macromolecular crowding on the size of a protein complex, SOD (superoxide dismutase). Crowding was induced using 400 MW PEG (polyethylene glycol),TEG (t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rajapaksha, Ajith, Stanley, Christopher B., Todd, Brian A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Biophysical Society 2015
Materias:	Proteins and Nucleic Acids
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336365/ https://www.ncbi.nlm.nih.gov/pubmed/25692601 http://dx.doi.org/10.1016/j.bpj.2014.12.046

Ejemplares similares

The effect of macromolecular crowding on single-round transcription by Escherichia coli RNA polymerase
por: Chung, SangYoon, et al.
Publicado: (2019)

The Complex Energy Landscape of the Protein IscU
por: Bothe, Jameson R., et al.
Publicado: (2015)

Effect of Oxidative Damage on the Stability and Dimerization of Superoxide Dismutase 1
por: Petrov, Drazen, et al.
Publicado: (2016)

Characterization of Folding Cores in the Cyclophilin A-Cyclosporin A Complex
por: Heal, Jack W., et al.
Publicado: (2015)

Oxidative Stress Alters the Morphology and Toxicity of Aortic Medial Amyloid
por: Davies, Hannah A., et al.
Publicado: (2015)

Functional Dynamics of Hexameric Helicase Probed by Hydrogen Exchange and Simulation
por: Radou, Gaël, et al.
Publicado: (2014)

Molecular Basis of the Mechanical Hierarchy in Myomesin Dimers for Sarcomere Integrity
por: Xiao, Senbo, et al.
Publicado: (2014)

Binding Affinities Controlled by Shifting Conformational Equilibria: Opportunities and Limitations
por: Michielssens, Servaas, et al.
Publicado: (2015)

Conformational Switching in a Light-Harvesting Protein as Followed by Single-Molecule Spectroscopy
por: Gall, Andrew, et al.
Publicado: (2015)

Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods
por: Shemilt, Laura, et al.
Publicado: (2015)

Ligand Binding Modulates the Structural Dynamics and Compactness of the Major Birch Pollen Allergen
por: Grutsch, Sarina, et al.
Publicado: (2014)

Characterization of the Conformational Fluctuations in the Josephin Domain of Ataxin-3
por: Sanfelice, Domenico, et al.
Publicado: (2014)

Direct Observation of Subunit Exchange along Mature Vimentin Intermediate Filaments
por: Nöding, Bernd, et al.
Publicado: (2014)

Quantifying the Entropy of Binding for Water Molecules in Protein Cavities by Computing Correlations
por: Huggins, David J.
Publicado: (2015)

Promiscuous Binding of Karyopherinβ1 Modulates FG Nucleoporin Barrier Function and Expedites NTF2 Transport Kinetics
por: Wagner, Raphael S., et al.
Publicado: (2015)

Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation
por: Moree, Ben, et al.
Publicado: (2015)

Revisiting the Anomalous Bending Elasticity of Sharply Bent DNA
por: Cong, Peiwen, et al.
Publicado: (2015)

Comprehensive Analysis of the Green-to-Blue Photoconversion of Full-Length Cyanobacteriochrome Tlr0924
por: Hardman, Samantha J.O., et al.
Publicado: (2014)

Kinesin-5 Allosteric Inhibitors Uncouple the Dynamics of Nucleotide, Microtubule, and Neck-Linker Binding Sites
por: Scarabelli, Guido, et al.
Publicado: (2014)

Distribution of Pico- and Nanosecond Motions in Disordered Proteins from Nuclear Spin Relaxation
por: Khan, Shahid N., et al.
Publicado: (2015)

Nascent β-Hairpin Formation of a Natively Unfolded Peptide Reveals the Role of Hydrophobic Contacts
por: Chen, Wei, et al.
Publicado: (2015)

Flavin Binding to the Deca-heme Cytochrome MtrC: Insights from Computational Molecular Simulation
por: Breuer, Marian, et al.
Publicado: (2015)

High-Affinity Interaction of the K-Ras4B Hypervariable Region with the Ras Active Site
por: Chavan, Tanmay S., et al.
Publicado: (2015)

RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding
por: Mazumder, Abhishek, et al.
Publicado: (2021)

Conformations of Flanking Bases in HIV-1 RNA DIS Kissing Complexes Studied by Molecular Dynamics
por: Réblová, Kamila, et al.
Publicado: (2007)

Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering
por: Sutton, Emma J., et al.
Publicado: (2018)

Purification, crystallization and X-ray structures of the two manganese superoxide dismutases from Caenorhabditis elegans
por: Trinh, Chi H., et al.
Publicado: (2008)

Superoxide Dismutase as a Novel Macromolecular Nitric Oxide Carrier: Preparation and Characterization
por: Chen, Ssu-Han, et al.
Publicado: (2012)

Investigating increasingly complex macromolecular systems with small-angle X-ray scattering
por: Vestergaard, Bente, et al.
Publicado: (2014)

Oligomeric complexes formed by Redβ single strand annealing protein in its different DNA bound states
por: Caldwell, Brian J, et al.
Publicado: (2021)

Components of variance in transcriptomics based on electrophoretic separation of cDNA fragments (cDNA-AFLP)
por: Weiberg, Arne, et al.
Publicado: (2009)

RNA pseudoknots
por: Westhof, Eric, et al.
Publicado: (1992)

Isometric artifacts from polymerase chain reaction‐massively parallel sequencing analysis of short tandem repeat loci: An emerging issue from a new technology?
por: Zupanič Pajnič, Irena, et al.
Publicado: (2022)

Superoxide Dismutases and Superoxide Reductases
por: Sheng, Yuewei, et al.
Publicado: (2014)

Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD–DNA complexes
por: Carter, Ashley R., et al.
Publicado: (2016)

YbeY is required for ribosome small subunit assembly and tRNA processing in human mitochondria
por: D’Souza, Aaron R, et al.
Publicado: (2021)

Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation
por: Kuznedelov, Konstantin, et al.
Publicado: (2016)

Crystal structures of Escherichia coli exonuclease I in complex with single-stranded DNA provide insights into the mechanism of processive digestion
por: Korada, Sai Krishna C., et al.
Publicado: (2013)

Immunodetection of human topoisomerase I-DNA covalent complexes
por: Patel, Anand G., et al.
Publicado: (2016)

A unique mRNA decapping complex in trypanosomes
por: Kramer, Susanne, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_f5pgp6iku4h9ghqtmqqil81ie6