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Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

BACKGROUND: Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this stai...

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Detalles Bibliográficos
Autores principales: Stiefel, Philipp, Schmidt-Emrich, Sabrina, Maniura-Weber, Katharina, Ren, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337318/
https://www.ncbi.nlm.nih.gov/pubmed/25881030
http://dx.doi.org/10.1186/s12866-015-0376-x