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Lipopolysaccharide induces expression of collagen VI in the rat lung

The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to...

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Autores principales: Okawa, Sayuri, Unuma, Kana, Yamada, Atsushi, Aki, Toshihiko, Uemura, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337498/
https://www.ncbi.nlm.nih.gov/pubmed/26023260
http://dx.doi.org/10.1293/tox.2014-0053
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author Okawa, Sayuri
Unuma, Kana
Yamada, Atsushi
Aki, Toshihiko
Uemura, Koichi
author_facet Okawa, Sayuri
Unuma, Kana
Yamada, Atsushi
Aki, Toshihiko
Uemura, Koichi
author_sort Okawa, Sayuri
collection PubMed
description The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage.
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spelling pubmed-43374982015-05-28 Lipopolysaccharide induces expression of collagen VI in the rat lung Okawa, Sayuri Unuma, Kana Yamada, Atsushi Aki, Toshihiko Uemura, Koichi J Toxicol Pathol Short Communication The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage. Japanese Society of Toxicologic Pathology 2014-12-27 2015-01 /pmc/articles/PMC4337498/ /pubmed/26023260 http://dx.doi.org/10.1293/tox.2014-0053 Text en ©2015 The Japanese Society of Toxicologic Pathology http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Short Communication
Okawa, Sayuri
Unuma, Kana
Yamada, Atsushi
Aki, Toshihiko
Uemura, Koichi
Lipopolysaccharide induces expression of collagen VI in the rat lung
title Lipopolysaccharide induces expression of collagen VI in the rat lung
title_full Lipopolysaccharide induces expression of collagen VI in the rat lung
title_fullStr Lipopolysaccharide induces expression of collagen VI in the rat lung
title_full_unstemmed Lipopolysaccharide induces expression of collagen VI in the rat lung
title_short Lipopolysaccharide induces expression of collagen VI in the rat lung
title_sort lipopolysaccharide induces expression of collagen vi in the rat lung
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337498/
https://www.ncbi.nlm.nih.gov/pubmed/26023260
http://dx.doi.org/10.1293/tox.2014-0053
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