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Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4

Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tu...

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Autores principales: Liu, Zhijing, Lu, Shi-Jiang, Lu, Yan, Tan, Xiaohua, Zhang, Xiaowei, Yang, Minlan, Zhang, Fuming, Li, Yulin, Quan, Chengshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337757/
https://www.ncbi.nlm.nih.gov/pubmed/25755671
http://dx.doi.org/10.1155/2015/389628
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author Liu, Zhijing
Lu, Shi-Jiang
Lu, Yan
Tan, Xiaohua
Zhang, Xiaowei
Yang, Minlan
Zhang, Fuming
Li, Yulin
Quan, Chengshi
author_facet Liu, Zhijing
Lu, Shi-Jiang
Lu, Yan
Tan, Xiaohua
Zhang, Xiaowei
Yang, Minlan
Zhang, Fuming
Li, Yulin
Quan, Chengshi
author_sort Liu, Zhijing
collection PubMed
description Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion.
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spelling pubmed-43377572015-03-09 Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 Liu, Zhijing Lu, Shi-Jiang Lu, Yan Tan, Xiaohua Zhang, Xiaowei Yang, Minlan Zhang, Fuming Li, Yulin Quan, Chengshi Stem Cells Int Research Article Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion. Hindawi Publishing Corporation 2015 2015-02-09 /pmc/articles/PMC4337757/ /pubmed/25755671 http://dx.doi.org/10.1155/2015/389628 Text en Copyright © 2015 Zhijing Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Zhijing
Lu, Shi-Jiang
Lu, Yan
Tan, Xiaohua
Zhang, Xiaowei
Yang, Minlan
Zhang, Fuming
Li, Yulin
Quan, Chengshi
Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title_full Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title_fullStr Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title_full_unstemmed Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title_short Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
title_sort transdifferentiation of human hair follicle mesenchymal stem cells into red blood cells by oct4
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337757/
https://www.ncbi.nlm.nih.gov/pubmed/25755671
http://dx.doi.org/10.1155/2015/389628
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