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Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337757/ https://www.ncbi.nlm.nih.gov/pubmed/25755671 http://dx.doi.org/10.1155/2015/389628 |
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author | Liu, Zhijing Lu, Shi-Jiang Lu, Yan Tan, Xiaohua Zhang, Xiaowei Yang, Minlan Zhang, Fuming Li, Yulin Quan, Chengshi |
author_facet | Liu, Zhijing Lu, Shi-Jiang Lu, Yan Tan, Xiaohua Zhang, Xiaowei Yang, Minlan Zhang, Fuming Li, Yulin Quan, Chengshi |
author_sort | Liu, Zhijing |
collection | PubMed |
description | Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion. |
format | Online Article Text |
id | pubmed-4337757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43377572015-03-09 Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 Liu, Zhijing Lu, Shi-Jiang Lu, Yan Tan, Xiaohua Zhang, Xiaowei Yang, Minlan Zhang, Fuming Li, Yulin Quan, Chengshi Stem Cells Int Research Article Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion. Hindawi Publishing Corporation 2015 2015-02-09 /pmc/articles/PMC4337757/ /pubmed/25755671 http://dx.doi.org/10.1155/2015/389628 Text en Copyright © 2015 Zhijing Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Zhijing Lu, Shi-Jiang Lu, Yan Tan, Xiaohua Zhang, Xiaowei Yang, Minlan Zhang, Fuming Li, Yulin Quan, Chengshi Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title | Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title_full | Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title_fullStr | Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title_full_unstemmed | Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title_short | Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4 |
title_sort | transdifferentiation of human hair follicle mesenchymal stem cells into red blood cells by oct4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337757/ https://www.ncbi.nlm.nih.gov/pubmed/25755671 http://dx.doi.org/10.1155/2015/389628 |
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