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Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma
Osteosarcoma is the most common primary bone tumor affecting children and young adults, and development of metastatic disease is associated with poor prognosis. The purpose of this study was to evaluate the antitumor efficacy of virotherapy with engineered measles virus (MV) vaccine strains in the t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337839/ https://www.ncbi.nlm.nih.gov/pubmed/25394505 http://dx.doi.org/10.1038/cgt.2014.54 |
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author | Musibay, Evidio Domingo Allen, Cory Kurokawa, Cheyne Hardcastle, Jayson J. Aderca, Ileana Msaouel, Pavlos Bansal, Aditya Jiang, Huailei DeGrado, Timothy R. Galanis, Evanthia |
author_facet | Musibay, Evidio Domingo Allen, Cory Kurokawa, Cheyne Hardcastle, Jayson J. Aderca, Ileana Msaouel, Pavlos Bansal, Aditya Jiang, Huailei DeGrado, Timothy R. Galanis, Evanthia |
author_sort | Musibay, Evidio Domingo |
collection | PubMed |
description | Osteosarcoma is the most common primary bone tumor affecting children and young adults, and development of metastatic disease is associated with poor prognosis. The purpose of this study was to evaluate the antitumor efficacy of virotherapy with engineered measles virus (MV) vaccine strains in the treatment of osteosarcoma. Cell lines derived from pediatric patients with osteosarcoma (HOS, MG63, 143B, KHOS-312H, U2-OS and SJSA1) were examined for MV-GFP and MV-NIS gene expression and cytotoxicity as defined by syncytial formation, cell death, and eradication of cell monolayers: significant antitumor activity was demonstrated. Findings were correlated with in vivo efficacy in subcutaneous, orthotopic (tibial bone), and lung metastatic osteosarcoma xenografts treated with the MV derivative MV-NIS via the intratumoral (IT) or intravenous (IV) route. Following treatment, we observed decrease in tumor growth of subcutaneous xenografts (p=0.0374) and prolongation of survival in mice with orthotopic (p<0.0001) and pulmonary metastatic osteosarcoma tumors (p=0.0207). Expression of the NIS transgene in MV-NIS infected tumors allowed for SPECT-CT and PET-CT imaging of virus infected tumors in vivo. Our data support the translational potential of MV-based virotherapy approaches in the treatment of recurrent and metastatic osteosarcoma. |
format | Online Article Text |
id | pubmed-4337839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-43378392015-05-14 Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma Musibay, Evidio Domingo Allen, Cory Kurokawa, Cheyne Hardcastle, Jayson J. Aderca, Ileana Msaouel, Pavlos Bansal, Aditya Jiang, Huailei DeGrado, Timothy R. Galanis, Evanthia Cancer Gene Ther Article Osteosarcoma is the most common primary bone tumor affecting children and young adults, and development of metastatic disease is associated with poor prognosis. The purpose of this study was to evaluate the antitumor efficacy of virotherapy with engineered measles virus (MV) vaccine strains in the treatment of osteosarcoma. Cell lines derived from pediatric patients with osteosarcoma (HOS, MG63, 143B, KHOS-312H, U2-OS and SJSA1) were examined for MV-GFP and MV-NIS gene expression and cytotoxicity as defined by syncytial formation, cell death, and eradication of cell monolayers: significant antitumor activity was demonstrated. Findings were correlated with in vivo efficacy in subcutaneous, orthotopic (tibial bone), and lung metastatic osteosarcoma xenografts treated with the MV derivative MV-NIS via the intratumoral (IT) or intravenous (IV) route. Following treatment, we observed decrease in tumor growth of subcutaneous xenografts (p=0.0374) and prolongation of survival in mice with orthotopic (p<0.0001) and pulmonary metastatic osteosarcoma tumors (p=0.0207). Expression of the NIS transgene in MV-NIS infected tumors allowed for SPECT-CT and PET-CT imaging of virus infected tumors in vivo. Our data support the translational potential of MV-based virotherapy approaches in the treatment of recurrent and metastatic osteosarcoma. 2014-11-14 2014-11 /pmc/articles/PMC4337839/ /pubmed/25394505 http://dx.doi.org/10.1038/cgt.2014.54 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Musibay, Evidio Domingo Allen, Cory Kurokawa, Cheyne Hardcastle, Jayson J. Aderca, Ileana Msaouel, Pavlos Bansal, Aditya Jiang, Huailei DeGrado, Timothy R. Galanis, Evanthia Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title | Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title_full | Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title_fullStr | Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title_full_unstemmed | Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title_short | Measles Edmonston Vaccine Strain Derivatives have Potent Oncolytic Activity against Osteosarcoma |
title_sort | measles edmonston vaccine strain derivatives have potent oncolytic activity against osteosarcoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337839/ https://www.ncbi.nlm.nih.gov/pubmed/25394505 http://dx.doi.org/10.1038/cgt.2014.54 |
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