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A Rapid and Convenient Method for Fluorescence Analysis of In Vitro Cultivated Metacestode Vesicles from Echinococcus multilocularis
We here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining...
Autores principales: | Cheng, Zhe, Liu, Fan, Zhu, Shan, Tian, Huimin, Wang, Liang, Wang, Yanhai |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337908/ https://www.ncbi.nlm.nih.gov/pubmed/25705880 http://dx.doi.org/10.1371/journal.pone.0118215 |
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