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Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability
Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338065/ https://www.ncbi.nlm.nih.gov/pubmed/25706563 http://dx.doi.org/10.1371/journal.pone.0116278 |
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author | Lee, Pei-Huan Liu, Chia-Ming Ho, Tzong-Shiann Tsai, Yi-Che Lin, Chi-Cheng Wang, Ya-Fang Chen, Yuh-Ling Yu, Chun-Keung Wang, Shih-Min Liu, Ching-Chuan Shiau, Ai-Li Lei, Huan-Yao Chang, Chih-Peng |
author_facet | Lee, Pei-Huan Liu, Chia-Ming Ho, Tzong-Shiann Tsai, Yi-Che Lin, Chi-Cheng Wang, Ya-Fang Chen, Yuh-Ling Yu, Chun-Keung Wang, Shih-Min Liu, Ching-Chuan Shiau, Ai-Li Lei, Huan-Yao Chang, Chih-Peng |
author_sort | Lee, Pei-Huan |
collection | PubMed |
description | Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to children’s health. EV71 infection is initiated by the attachment of the virion to the target cell surface. Although this process relies primarily upon interaction between viruses and cell surface receptors, soluble factors may also influence the binding of EV71 to host cells.Galectin-1 has been reported to participate in several virus infections, but is not addressed in EV71. In this study, we found that the serum levels of galectin-1 in EV71-infected children were higher than those in non-infected people. In EV71 infected cells, galectin-1 was found to be associated with the EV71 VP1 and VP3 via carbohydrate residues and subsequently released and bound to another cell surface along with the virus. EV71 propagated from galectin-1 knockdown SK-N-SH cells exhibited lower infectivity in cultured cells and less pathogenicity in mice than the virus propagated from parental cells. In addition, this galectin-1-free EV71 virus was sensitive to high temperature and lost its viability after long-term storage, which could be restored following supplement of recombinant galectin-1. Taken together, our findings uncover a new role of galectin-1 in facilitating EV71 virus infection. |
format | Online Article Text |
id | pubmed-4338065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43380652015-03-04 Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability Lee, Pei-Huan Liu, Chia-Ming Ho, Tzong-Shiann Tsai, Yi-Che Lin, Chi-Cheng Wang, Ya-Fang Chen, Yuh-Ling Yu, Chun-Keung Wang, Shih-Min Liu, Ching-Chuan Shiau, Ai-Li Lei, Huan-Yao Chang, Chih-Peng PLoS One Research Article Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to children’s health. EV71 infection is initiated by the attachment of the virion to the target cell surface. Although this process relies primarily upon interaction between viruses and cell surface receptors, soluble factors may also influence the binding of EV71 to host cells.Galectin-1 has been reported to participate in several virus infections, but is not addressed in EV71. In this study, we found that the serum levels of galectin-1 in EV71-infected children were higher than those in non-infected people. In EV71 infected cells, galectin-1 was found to be associated with the EV71 VP1 and VP3 via carbohydrate residues and subsequently released and bound to another cell surface along with the virus. EV71 propagated from galectin-1 knockdown SK-N-SH cells exhibited lower infectivity in cultured cells and less pathogenicity in mice than the virus propagated from parental cells. In addition, this galectin-1-free EV71 virus was sensitive to high temperature and lost its viability after long-term storage, which could be restored following supplement of recombinant galectin-1. Taken together, our findings uncover a new role of galectin-1 in facilitating EV71 virus infection. Public Library of Science 2015-02-23 /pmc/articles/PMC4338065/ /pubmed/25706563 http://dx.doi.org/10.1371/journal.pone.0116278 Text en © 2015 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lee, Pei-Huan Liu, Chia-Ming Ho, Tzong-Shiann Tsai, Yi-Che Lin, Chi-Cheng Wang, Ya-Fang Chen, Yuh-Ling Yu, Chun-Keung Wang, Shih-Min Liu, Ching-Chuan Shiau, Ai-Li Lei, Huan-Yao Chang, Chih-Peng Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title | Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title_full | Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title_fullStr | Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title_full_unstemmed | Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title_short | Enterovirus 71 Virion-Associated Galectin-1 Facilitates Viral Replication and Stability |
title_sort | enterovirus 71 virion-associated galectin-1 facilitates viral replication and stability |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338065/ https://www.ncbi.nlm.nih.gov/pubmed/25706563 http://dx.doi.org/10.1371/journal.pone.0116278 |
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