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Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models
We aimed to compare [(18)F]-florbetaben PET imaging in four transgenic mouse strains modelling Alzheimer’s disease (AD), with the main focus on APPswe/PS2 mice and C57Bl/6 mice serving as controls (WT). A consistent PET protocol (N = 82 PET scans) was used, with cortical standardized uptake value ra...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338066/ https://www.ncbi.nlm.nih.gov/pubmed/25706990 http://dx.doi.org/10.1371/journal.pone.0116678 |
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author | Brendel, Matthias Jaworska, Anna Grießinger, Eric Rötzer, Christina Burgold, Steffen Gildehaus, Franz-Josef Carlsen, Janette Cumming, Paul Baumann, Karlheinz Haass, Christian Steiner, Harald Bartenstein, Peter Herms, Jochen Rominger, Axel |
author_facet | Brendel, Matthias Jaworska, Anna Grießinger, Eric Rötzer, Christina Burgold, Steffen Gildehaus, Franz-Josef Carlsen, Janette Cumming, Paul Baumann, Karlheinz Haass, Christian Steiner, Harald Bartenstein, Peter Herms, Jochen Rominger, Axel |
author_sort | Brendel, Matthias |
collection | PubMed |
description | We aimed to compare [(18)F]-florbetaben PET imaging in four transgenic mouse strains modelling Alzheimer’s disease (AD), with the main focus on APPswe/PS2 mice and C57Bl/6 mice serving as controls (WT). A consistent PET protocol (N = 82 PET scans) was used, with cortical standardized uptake value ratio (SUVR) relative to cerebellum as the endpoint. We correlated methoxy-X04 staining of β-amyloid with PET results, and undertook ex vivo autoradiography for further validation of a partial volume effect correction (PVEC) of PET data. The SUVR in APPswe/PS2 increased from 0.95±0.04 at five months (N = 5) and 1.04±0.03 (p<0.05) at eight months (N = 7) to 1.07±0.04 (p<0.005) at ten months (N = 6), 1.28±0.06 (p<0.001) at 16 months (N = 6) and 1.39±0.09 (p<0.001) at 19 months (N = 6). SUVR was 0.95±0.03 in WT mice of all ages (N = 22). In APPswe/PS1G384A mice, the SUVR was 0.93/0.98 at five months (N = 2) and 1.11 at 16 months (N = 1). In APPswe/PS1dE9 mice, the SUVR declined from 0.96/0.96 at 12 months (N = 2) to 0.91/0.92 at 24 months (N = 2), due to β-amyloid plaques in cerebellum. PVEC reduced the discrepancy between SUVR-PET and autoradiography from −22% to +2% and increased the differences between young and aged transgenic animals. SUVR and plaque load correlated highly between strains for uncorrected (R = 0.94, p<0.001) and PVE-corrected (R = 0.95, p<0.001) data. We find that APPswe/PS2 mice may be optimal for longitudinal amyloid-PET monitoring in planned interventions studies. |
format | Online Article Text |
id | pubmed-4338066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43380662015-03-04 Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models Brendel, Matthias Jaworska, Anna Grießinger, Eric Rötzer, Christina Burgold, Steffen Gildehaus, Franz-Josef Carlsen, Janette Cumming, Paul Baumann, Karlheinz Haass, Christian Steiner, Harald Bartenstein, Peter Herms, Jochen Rominger, Axel PLoS One Research Article We aimed to compare [(18)F]-florbetaben PET imaging in four transgenic mouse strains modelling Alzheimer’s disease (AD), with the main focus on APPswe/PS2 mice and C57Bl/6 mice serving as controls (WT). A consistent PET protocol (N = 82 PET scans) was used, with cortical standardized uptake value ratio (SUVR) relative to cerebellum as the endpoint. We correlated methoxy-X04 staining of β-amyloid with PET results, and undertook ex vivo autoradiography for further validation of a partial volume effect correction (PVEC) of PET data. The SUVR in APPswe/PS2 increased from 0.95±0.04 at five months (N = 5) and 1.04±0.03 (p<0.05) at eight months (N = 7) to 1.07±0.04 (p<0.005) at ten months (N = 6), 1.28±0.06 (p<0.001) at 16 months (N = 6) and 1.39±0.09 (p<0.001) at 19 months (N = 6). SUVR was 0.95±0.03 in WT mice of all ages (N = 22). In APPswe/PS1G384A mice, the SUVR was 0.93/0.98 at five months (N = 2) and 1.11 at 16 months (N = 1). In APPswe/PS1dE9 mice, the SUVR declined from 0.96/0.96 at 12 months (N = 2) to 0.91/0.92 at 24 months (N = 2), due to β-amyloid plaques in cerebellum. PVEC reduced the discrepancy between SUVR-PET and autoradiography from −22% to +2% and increased the differences between young and aged transgenic animals. SUVR and plaque load correlated highly between strains for uncorrected (R = 0.94, p<0.001) and PVE-corrected (R = 0.95, p<0.001) data. We find that APPswe/PS2 mice may be optimal for longitudinal amyloid-PET monitoring in planned interventions studies. Public Library of Science 2015-02-23 /pmc/articles/PMC4338066/ /pubmed/25706990 http://dx.doi.org/10.1371/journal.pone.0116678 Text en © 2015 Brendel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Brendel, Matthias Jaworska, Anna Grießinger, Eric Rötzer, Christina Burgold, Steffen Gildehaus, Franz-Josef Carlsen, Janette Cumming, Paul Baumann, Karlheinz Haass, Christian Steiner, Harald Bartenstein, Peter Herms, Jochen Rominger, Axel Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title | Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title_full | Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title_fullStr | Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title_full_unstemmed | Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title_short | Cross-Sectional Comparison of Small Animal [(18)F]-Florbetaben Amyloid-PET between Transgenic AD Mouse Models |
title_sort | cross-sectional comparison of small animal [(18)f]-florbetaben amyloid-pet between transgenic ad mouse models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338066/ https://www.ncbi.nlm.nih.gov/pubmed/25706990 http://dx.doi.org/10.1371/journal.pone.0116678 |
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