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SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT

The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma...

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Autores principales: Wan, Feng, Cheng, Chao, Wang, Zongwei, Xiao, Xingyuan, Zeng, Hanqing, Xing, Shian, Chen, Xuepan, Wang, Jin, Li, Sen, Zhang, Youpeng, Xiang, Wei, Zhu, Zhineng, Johnson, Cameron, Zhu, Zhaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338074/
https://www.ncbi.nlm.nih.gov/pubmed/25706386
http://dx.doi.org/10.1371/journal.pone.0117518
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author Wan, Feng
Cheng, Chao
Wang, Zongwei
Xiao, Xingyuan
Zeng, Hanqing
Xing, Shian
Chen, Xuepan
Wang, Jin
Li, Sen
Zhang, Youpeng
Xiang, Wei
Zhu, Zhineng
Johnson, Cameron
Zhu, Zhaohui
author_facet Wan, Feng
Cheng, Chao
Wang, Zongwei
Xiao, Xingyuan
Zeng, Hanqing
Xing, Shian
Chen, Xuepan
Wang, Jin
Li, Sen
Zhang, Youpeng
Xiang, Wei
Zhu, Zhineng
Johnson, Cameron
Zhu, Zhaohui
author_sort Wan, Feng
collection PubMed
description The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma (BTCC) and further elucidate if this effect works through an epithelial-mesenchymal transition (EMT) pathway. The expression of SATB1, E-cadherin (epithelial markers), vimentin (mesenchymal markers) in BTCC tissues and adjacent noncancerous tissues, as well as in two cell lines of bladder cancer were investigated. Whether the SATB1 expression is associated with clinicopathological factors or not was statistically analyzed. Cell invasion and migration, cell cycle, cell proliferation and apoptosis were evaluated in SATB1 knockdown and overexpressed cell lines. Our results showed that the expression of SATB1 was remarkably up-regulated both in BTCC tissues and in bladder cancer cell lines with high potential of metastasis. The results were also associated with EMT markers and poor prognosis of BTCC patients. Moreover, SATB1 induced EMT processes through downregulation of E-cadherin, upregulation of E-cadherin repressors (Snail, Slug and vimentin). SATB1 also promoted cell cycle progression, cell proliferation, cell invasion and cell migration, but did not alter cell survival. In conclusion, our results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes. Further, it may be a novel therapeutic target for aggressive bladder cancers.
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spelling pubmed-43380742015-03-04 SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT Wan, Feng Cheng, Chao Wang, Zongwei Xiao, Xingyuan Zeng, Hanqing Xing, Shian Chen, Xuepan Wang, Jin Li, Sen Zhang, Youpeng Xiang, Wei Zhu, Zhineng Johnson, Cameron Zhu, Zhaohui PLoS One Research Article The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma (BTCC) and further elucidate if this effect works through an epithelial-mesenchymal transition (EMT) pathway. The expression of SATB1, E-cadherin (epithelial markers), vimentin (mesenchymal markers) in BTCC tissues and adjacent noncancerous tissues, as well as in two cell lines of bladder cancer were investigated. Whether the SATB1 expression is associated with clinicopathological factors or not was statistically analyzed. Cell invasion and migration, cell cycle, cell proliferation and apoptosis were evaluated in SATB1 knockdown and overexpressed cell lines. Our results showed that the expression of SATB1 was remarkably up-regulated both in BTCC tissues and in bladder cancer cell lines with high potential of metastasis. The results were also associated with EMT markers and poor prognosis of BTCC patients. Moreover, SATB1 induced EMT processes through downregulation of E-cadherin, upregulation of E-cadherin repressors (Snail, Slug and vimentin). SATB1 also promoted cell cycle progression, cell proliferation, cell invasion and cell migration, but did not alter cell survival. In conclusion, our results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes. Further, it may be a novel therapeutic target for aggressive bladder cancers. Public Library of Science 2015-02-23 /pmc/articles/PMC4338074/ /pubmed/25706386 http://dx.doi.org/10.1371/journal.pone.0117518 Text en © 2015 Wan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wan, Feng
Cheng, Chao
Wang, Zongwei
Xiao, Xingyuan
Zeng, Hanqing
Xing, Shian
Chen, Xuepan
Wang, Jin
Li, Sen
Zhang, Youpeng
Xiang, Wei
Zhu, Zhineng
Johnson, Cameron
Zhu, Zhaohui
SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title_full SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title_fullStr SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title_full_unstemmed SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title_short SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
title_sort satb1 overexpression regulates the development and progression in bladder cancer through emt
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338074/
https://www.ncbi.nlm.nih.gov/pubmed/25706386
http://dx.doi.org/10.1371/journal.pone.0117518
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