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Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans

In order to understand the mechanism governing radiation resistance in Deinococcus radiodurans, current efforts are aimed at identifying potential candidates from a large repertoire of unique Deinococcal genes and protein families. DR0053 belongs to the DinB/YfiT protein family, which is an over-rep...

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Autores principales: Appukuttan, Deepti, Seo, Ho Seong, Jeong, Sunwook, Im, Sunghun, Joe, Minho, Song, Dusup, Choi, Jungjoon, Lim, Sangyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338110/
https://www.ncbi.nlm.nih.gov/pubmed/25706748
http://dx.doi.org/10.1371/journal.pone.0118275
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author Appukuttan, Deepti
Seo, Ho Seong
Jeong, Sunwook
Im, Sunghun
Joe, Minho
Song, Dusup
Choi, Jungjoon
Lim, Sangyong
author_facet Appukuttan, Deepti
Seo, Ho Seong
Jeong, Sunwook
Im, Sunghun
Joe, Minho
Song, Dusup
Choi, Jungjoon
Lim, Sangyong
author_sort Appukuttan, Deepti
collection PubMed
description In order to understand the mechanism governing radiation resistance in Deinococcus radiodurans, current efforts are aimed at identifying potential candidates from a large repertoire of unique Deinococcal genes and protein families. DR0053 belongs to the DinB/YfiT protein family, which is an over-represented protein family in D. radiodurans. We observed that dr0053 transcript levels were highly induced in response to gamma radiation (γ-radiation) and mitomycin C (MMC) exposure depending on PprI, RecA and the DrtR/S two-component signal transduction system. Protein profiles demonstrated that DR0053 is a highly induced protein in cultures exposed to 10 kGy γ-radiation. We were able to determine the transcriptional start site of dr0053, which was induced upon irradiation, and to assign the 133-bp promoter region of dr0053 as essential for radiation responsiveness through primer extension and promoter deletion analyses. A dr0053 mutant strain displayed sensitivity to γ-radiation and MMC exposure, but not hydrogen peroxide, suggesting that DR0053 helps cells recover from DNA damage. Bioinformatic analyses revealed that DR0053 is similar to the Bacillus subtilis protein YjoA, which is a substrate of bacterial protein-tyrosine kinases. Taken together, the DNA damage-inducible (din) gene dr0053 may be regulated at the transcriptional and post-translational levels.
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spelling pubmed-43381102015-03-04 Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans Appukuttan, Deepti Seo, Ho Seong Jeong, Sunwook Im, Sunghun Joe, Minho Song, Dusup Choi, Jungjoon Lim, Sangyong PLoS One Research Article In order to understand the mechanism governing radiation resistance in Deinococcus radiodurans, current efforts are aimed at identifying potential candidates from a large repertoire of unique Deinococcal genes and protein families. DR0053 belongs to the DinB/YfiT protein family, which is an over-represented protein family in D. radiodurans. We observed that dr0053 transcript levels were highly induced in response to gamma radiation (γ-radiation) and mitomycin C (MMC) exposure depending on PprI, RecA and the DrtR/S two-component signal transduction system. Protein profiles demonstrated that DR0053 is a highly induced protein in cultures exposed to 10 kGy γ-radiation. We were able to determine the transcriptional start site of dr0053, which was induced upon irradiation, and to assign the 133-bp promoter region of dr0053 as essential for radiation responsiveness through primer extension and promoter deletion analyses. A dr0053 mutant strain displayed sensitivity to γ-radiation and MMC exposure, but not hydrogen peroxide, suggesting that DR0053 helps cells recover from DNA damage. Bioinformatic analyses revealed that DR0053 is similar to the Bacillus subtilis protein YjoA, which is a substrate of bacterial protein-tyrosine kinases. Taken together, the DNA damage-inducible (din) gene dr0053 may be regulated at the transcriptional and post-translational levels. Public Library of Science 2015-02-23 /pmc/articles/PMC4338110/ /pubmed/25706748 http://dx.doi.org/10.1371/journal.pone.0118275 Text en © 2015 Appukuttan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Appukuttan, Deepti
Seo, Ho Seong
Jeong, Sunwook
Im, Sunghun
Joe, Minho
Song, Dusup
Choi, Jungjoon
Lim, Sangyong
Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title_full Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title_fullStr Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title_full_unstemmed Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title_short Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans
title_sort expression and mutational analysis of dinb-like protein dr0053 in deinococcus radiodurans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338110/
https://www.ncbi.nlm.nih.gov/pubmed/25706748
http://dx.doi.org/10.1371/journal.pone.0118275
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