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Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood

OBJECTIVES: Phthalates might be implicated with obesity and insulin sensitivity. We evaluated the levels of primary and secondary metabolites of Di-(2-ethylhexyl) phthalate (DEHP) in urine in obese and normal-weight subjects both before and during puberty, and investigated their relationships with a...

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Autores principales: Smerieri, Arianna, Testa, Chiara, Lazzeroni, Pietro, Nuti, Francesca, Grossi, Enzo, Cesari, Silvia, Montanini, Luisa, Latini, Giuseppe, Bernasconi, Sergio, Papini, Anna Maria, Street, Maria E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338209/
https://www.ncbi.nlm.nih.gov/pubmed/25706863
http://dx.doi.org/10.1371/journal.pone.0117831
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author Smerieri, Arianna
Testa, Chiara
Lazzeroni, Pietro
Nuti, Francesca
Grossi, Enzo
Cesari, Silvia
Montanini, Luisa
Latini, Giuseppe
Bernasconi, Sergio
Papini, Anna Maria
Street, Maria E.
author_facet Smerieri, Arianna
Testa, Chiara
Lazzeroni, Pietro
Nuti, Francesca
Grossi, Enzo
Cesari, Silvia
Montanini, Luisa
Latini, Giuseppe
Bernasconi, Sergio
Papini, Anna Maria
Street, Maria E.
author_sort Smerieri, Arianna
collection PubMed
description OBJECTIVES: Phthalates might be implicated with obesity and insulin sensitivity. We evaluated the levels of primary and secondary metabolites of Di-(2-ethylhexyl) phthalate (DEHP) in urine in obese and normal-weight subjects both before and during puberty, and investigated their relationships with auxological parameters and indexes of insulin sensitivity. DESIGN AND METHODS: DEHP metabolites (MEHP, 6-OH-MEHP, 5-oxo-MEHP, 5-OH-MEHP, and 5-CX-MEHP), were measured in urine by RP-HPLC-ESI-MS. Traditional statistical analysis and a data mining analysis using the Auto-CM analysis were able to offer an insight into the complex biological connections between the studied variables. RESULTS: The data showed changes in DEHP metabolites in urine related with obesity, puberty, and presence of insulin resistance. Changes in urine metabolites were related with age, height and weight, waist circumference and waist to height ratio, thus to fat distribution. In addition, clear relationships in both obese and normal-weight subjects were detected among MEHP, its products of oxidation and measurements of insulin sensitivity. CONCLUSION: It remains to be elucidated whether exposure to phthalates per se is actually the risk factor or if the ability of the body to metabolize phthalates is actually the key point. Further studies that span from conception to elderly subjects besides further understanding of DEHP metabolism are warranted to clarify these aspects.
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spelling pubmed-43382092015-03-04 Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood Smerieri, Arianna Testa, Chiara Lazzeroni, Pietro Nuti, Francesca Grossi, Enzo Cesari, Silvia Montanini, Luisa Latini, Giuseppe Bernasconi, Sergio Papini, Anna Maria Street, Maria E. PLoS One Research Article OBJECTIVES: Phthalates might be implicated with obesity and insulin sensitivity. We evaluated the levels of primary and secondary metabolites of Di-(2-ethylhexyl) phthalate (DEHP) in urine in obese and normal-weight subjects both before and during puberty, and investigated their relationships with auxological parameters and indexes of insulin sensitivity. DESIGN AND METHODS: DEHP metabolites (MEHP, 6-OH-MEHP, 5-oxo-MEHP, 5-OH-MEHP, and 5-CX-MEHP), were measured in urine by RP-HPLC-ESI-MS. Traditional statistical analysis and a data mining analysis using the Auto-CM analysis were able to offer an insight into the complex biological connections between the studied variables. RESULTS: The data showed changes in DEHP metabolites in urine related with obesity, puberty, and presence of insulin resistance. Changes in urine metabolites were related with age, height and weight, waist circumference and waist to height ratio, thus to fat distribution. In addition, clear relationships in both obese and normal-weight subjects were detected among MEHP, its products of oxidation and measurements of insulin sensitivity. CONCLUSION: It remains to be elucidated whether exposure to phthalates per se is actually the risk factor or if the ability of the body to metabolize phthalates is actually the key point. Further studies that span from conception to elderly subjects besides further understanding of DEHP metabolism are warranted to clarify these aspects. Public Library of Science 2015-02-23 /pmc/articles/PMC4338209/ /pubmed/25706863 http://dx.doi.org/10.1371/journal.pone.0117831 Text en © 2015 Smerieri et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Smerieri, Arianna
Testa, Chiara
Lazzeroni, Pietro
Nuti, Francesca
Grossi, Enzo
Cesari, Silvia
Montanini, Luisa
Latini, Giuseppe
Bernasconi, Sergio
Papini, Anna Maria
Street, Maria E.
Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title_full Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title_fullStr Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title_full_unstemmed Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title_short Di-(2-Ethylhexyl) Phthalate Metabolites in Urine Show Age-Related Changes and Associations with Adiposity and Parameters of Insulin Sensitivity in Childhood
title_sort di-(2-ethylhexyl) phthalate metabolites in urine show age-related changes and associations with adiposity and parameters of insulin sensitivity in childhood
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338209/
https://www.ncbi.nlm.nih.gov/pubmed/25706863
http://dx.doi.org/10.1371/journal.pone.0117831
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