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Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O(2) (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O(2) were characterized. Methane production and protein conten...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338226/ https://www.ncbi.nlm.nih.gov/pubmed/25706146 http://dx.doi.org/10.1371/journal.pone.0117331 |
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author | Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael |
author_facet | Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael |
author_sort | Jasso-Chávez, Ricardo |
collection | PubMed |
description | Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O(2) (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O(2) were characterized. Methane production and protein content, as indicators of cell growth, did not change in air-adapted cells respect to cells cultured under anoxia (control cells). In contrast, growth and methane production significantly decreased in control cells exposed for the first time to O(2). Production of reactive oxygen species was 50 times lower in air-adapted cells versus control cells, suggesting enhanced anti-oxidant mechanisms that attenuated the O(2) toxicity. In this regard, (i) the transcripts and activities of superoxide dismutase, catalase and peroxidase significantly increased; and (ii) the thiol-molecules (cysteine + coenzyme M-SH + sulfide) and polyphosphate contents were respectively 2 and 5 times higher in air-adapted cells versus anaerobic-control cells. Long-term cultures (18 days) of air-adapted cells exposed to 2% O(2) exhibited the ability to form biofilms. These data indicate that M. acetivorans develops multiple mechanisms to contend with O(2) and the associated oxidative stress, as also suggested by genome analyses for some methanogens. |
format | Online Article Text |
id | pubmed-4338226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43382262015-03-04 Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael PLoS One Research Article Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O(2) (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O(2) were characterized. Methane production and protein content, as indicators of cell growth, did not change in air-adapted cells respect to cells cultured under anoxia (control cells). In contrast, growth and methane production significantly decreased in control cells exposed for the first time to O(2). Production of reactive oxygen species was 50 times lower in air-adapted cells versus control cells, suggesting enhanced anti-oxidant mechanisms that attenuated the O(2) toxicity. In this regard, (i) the transcripts and activities of superoxide dismutase, catalase and peroxidase significantly increased; and (ii) the thiol-molecules (cysteine + coenzyme M-SH + sulfide) and polyphosphate contents were respectively 2 and 5 times higher in air-adapted cells versus anaerobic-control cells. Long-term cultures (18 days) of air-adapted cells exposed to 2% O(2) exhibited the ability to form biofilms. These data indicate that M. acetivorans develops multiple mechanisms to contend with O(2) and the associated oxidative stress, as also suggested by genome analyses for some methanogens. Public Library of Science 2015-02-23 /pmc/articles/PMC4338226/ /pubmed/25706146 http://dx.doi.org/10.1371/journal.pone.0117331 Text en © 2015 Jasso-Chávez et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title | Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title_full | Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title_fullStr | Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title_full_unstemmed | Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title_short | Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress |
title_sort | air-adapted methanosarcina acetivorans shows high methane production and develops resistance against oxygen stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338226/ https://www.ncbi.nlm.nih.gov/pubmed/25706146 http://dx.doi.org/10.1371/journal.pone.0117331 |
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