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Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns
Intron retention is one of the least studied forms of alternative splicing. Through the use of retrovirus and other model systems, it was established many years ago that mRNAs with retained introns are subject to restriction both at the level of nucleocytoplasmic export and cytoplasmic expression. I...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338338/ https://www.ncbi.nlm.nih.gov/pubmed/25605961 http://dx.doi.org/10.1261/rna.048520.114 |
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author | Wang, Baomin Rekosh, David Hammarskjold, Marie-Louise |
author_facet | Wang, Baomin Rekosh, David Hammarskjold, Marie-Louise |
author_sort | Wang, Baomin |
collection | PubMed |
description | Intron retention is one of the least studied forms of alternative splicing. Through the use of retrovirus and other model systems, it was established many years ago that mRNAs with retained introns are subject to restriction both at the level of nucleocytoplasmic export and cytoplasmic expression. It was also demonstrated that specific cis-acting elements in the mRNA could serve to bypass these restrictions. Here we show that one of these elements, the constitutive transport element (CTE), first identified in the retrovirus MPMV and subsequently in the human NXF1 gene, is a highly conserved element. Using GERP analysis, CTEs with strong primary sequence homology, predicted to display identical secondary structure, were identified in NXF genes from >30 mammalian species. CTEs were also identified in the predicted NXF1 genes of zebrafish and coelacanths. The CTE from the zebrafish NXF1 was shown to function efficiently to achieve expression of mRNA with a retained intron in human cells in conjunction with zebrafish Nxf1 and cofactor Nxt proteins. This demonstrates that all essential functional components for expression of mRNA with retained introns have been conserved from fish to man. |
format | Online Article Text |
id | pubmed-4338338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43383382016-03-01 Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns Wang, Baomin Rekosh, David Hammarskjold, Marie-Louise RNA Articles Intron retention is one of the least studied forms of alternative splicing. Through the use of retrovirus and other model systems, it was established many years ago that mRNAs with retained introns are subject to restriction both at the level of nucleocytoplasmic export and cytoplasmic expression. It was also demonstrated that specific cis-acting elements in the mRNA could serve to bypass these restrictions. Here we show that one of these elements, the constitutive transport element (CTE), first identified in the retrovirus MPMV and subsequently in the human NXF1 gene, is a highly conserved element. Using GERP analysis, CTEs with strong primary sequence homology, predicted to display identical secondary structure, were identified in NXF genes from >30 mammalian species. CTEs were also identified in the predicted NXF1 genes of zebrafish and coelacanths. The CTE from the zebrafish NXF1 was shown to function efficiently to achieve expression of mRNA with a retained intron in human cells in conjunction with zebrafish Nxf1 and cofactor Nxt proteins. This demonstrates that all essential functional components for expression of mRNA with retained introns have been conserved from fish to man. Cold Spring Harbor Laboratory Press 2015-03 /pmc/articles/PMC4338338/ /pubmed/25605961 http://dx.doi.org/10.1261/rna.048520.114 Text en © 2015 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Articles Wang, Baomin Rekosh, David Hammarskjold, Marie-Louise Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title | Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title_full | Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title_fullStr | Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title_full_unstemmed | Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title_short | Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns |
title_sort | evolutionary conservation of a molecular machinery for export and expression of mrnas with retained introns |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338338/ https://www.ncbi.nlm.nih.gov/pubmed/25605961 http://dx.doi.org/10.1261/rna.048520.114 |
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