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Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults

The objective of this study was to compare quinolone resistance and gyrA mutations in clinical isolates of Klebsiella pneumoniae and Escherichia coli from Chinese adults who used quinolone in the preceding month and children without any known history of quinolone administration. The antimicrobial su...

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Autores principales: Huang, Ying, Ogutu, James O., Gu, Jiarui, Ding, Fengshu, You, Yuhong, Huo, Yan, Zhao, Hong, Li, Wenjing, Zhang, Zhiwei, Zhang, Wenli, Chen, Xiaobei, Fu, Yingmei, Zhang, Fengmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338376/
https://www.ncbi.nlm.nih.gov/pubmed/25756041
http://dx.doi.org/10.1155/2015/168292
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author Huang, Ying
Ogutu, James O.
Gu, Jiarui
Ding, Fengshu
You, Yuhong
Huo, Yan
Zhao, Hong
Li, Wenjing
Zhang, Zhiwei
Zhang, Wenli
Chen, Xiaobei
Fu, Yingmei
Zhang, Fengmin
author_facet Huang, Ying
Ogutu, James O.
Gu, Jiarui
Ding, Fengshu
You, Yuhong
Huo, Yan
Zhao, Hong
Li, Wenjing
Zhang, Zhiwei
Zhang, Wenli
Chen, Xiaobei
Fu, Yingmei
Zhang, Fengmin
author_sort Huang, Ying
collection PubMed
description The objective of this study was to compare quinolone resistance and gyrA mutations in clinical isolates of Klebsiella pneumoniae and Escherichia coli from Chinese adults who used quinolone in the preceding month and children without any known history of quinolone administration. The antimicrobial susceptibilities of 61 isolates from children and 79 isolates from adults were determined. The mutations in the quinolone resistance-determining regions in gyrA gene were detected by PCR and DNA sequencing. Fluoroquinolone resistance and types of gyrA mutations in isolates from children and adults were compared and statistically analyzed. No significant differences were detected in the resistance rates of ciprofloxacin and levofloxacin between children and adults among isolates of the two species (all P > 0.05). The double mutation Ser83→Leu + Asp87→Asn in the ciprofloxacin-resistant isolates occurred in 73.7% isolates from the children and 67.9% from the adults, respectively (P = 0.5444). Children with no known history of quinolone administration were found to carry fluoroquinolone-resistant Enterobacteriaceae isolates. The occurrence of ciprofloxacin resistance and the major types of gyrA mutations in the isolates from the children were similar to those from adults. The results indicate that precautions should be taken on environmental issues resulting from widespread transmission of quinolone resistance.
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spelling pubmed-43383762015-03-09 Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults Huang, Ying Ogutu, James O. Gu, Jiarui Ding, Fengshu You, Yuhong Huo, Yan Zhao, Hong Li, Wenjing Zhang, Zhiwei Zhang, Wenli Chen, Xiaobei Fu, Yingmei Zhang, Fengmin Biomed Res Int Research Article The objective of this study was to compare quinolone resistance and gyrA mutations in clinical isolates of Klebsiella pneumoniae and Escherichia coli from Chinese adults who used quinolone in the preceding month and children without any known history of quinolone administration. The antimicrobial susceptibilities of 61 isolates from children and 79 isolates from adults were determined. The mutations in the quinolone resistance-determining regions in gyrA gene were detected by PCR and DNA sequencing. Fluoroquinolone resistance and types of gyrA mutations in isolates from children and adults were compared and statistically analyzed. No significant differences were detected in the resistance rates of ciprofloxacin and levofloxacin between children and adults among isolates of the two species (all P > 0.05). The double mutation Ser83→Leu + Asp87→Asn in the ciprofloxacin-resistant isolates occurred in 73.7% isolates from the children and 67.9% from the adults, respectively (P = 0.5444). Children with no known history of quinolone administration were found to carry fluoroquinolone-resistant Enterobacteriaceae isolates. The occurrence of ciprofloxacin resistance and the major types of gyrA mutations in the isolates from the children were similar to those from adults. The results indicate that precautions should be taken on environmental issues resulting from widespread transmission of quinolone resistance. Hindawi Publishing Corporation 2015 2015-02-10 /pmc/articles/PMC4338376/ /pubmed/25756041 http://dx.doi.org/10.1155/2015/168292 Text en Copyright © 2015 Ying Huang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Ying
Ogutu, James O.
Gu, Jiarui
Ding, Fengshu
You, Yuhong
Huo, Yan
Zhao, Hong
Li, Wenjing
Zhang, Zhiwei
Zhang, Wenli
Chen, Xiaobei
Fu, Yingmei
Zhang, Fengmin
Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title_full Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title_fullStr Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title_full_unstemmed Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title_short Comparative Analysis of Quinolone Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from Chinese Children and Adults
title_sort comparative analysis of quinolone resistance in clinical isolates of klebsiella pneumoniae and escherichia coli from chinese children and adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338376/
https://www.ncbi.nlm.nih.gov/pubmed/25756041
http://dx.doi.org/10.1155/2015/168292
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