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Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis

Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression st...

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Autores principales: Wolfram, Dolores, Morandi, Evi M., Eberhart, Nadine, Hautz, Theresa, Hackl, Hubert, Zelger, Bettina, Riede, Gregor, Wachter, Tanja, Dubrac, Sandrine, Ploner, Christian, Pierer, Gerhard, Schneeberger, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338383/
https://www.ncbi.nlm.nih.gov/pubmed/25756043
http://dx.doi.org/10.1155/2015/259160
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author Wolfram, Dolores
Morandi, Evi M.
Eberhart, Nadine
Hautz, Theresa
Hackl, Hubert
Zelger, Bettina
Riede, Gregor
Wachter, Tanja
Dubrac, Sandrine
Ploner, Christian
Pierer, Gerhard
Schneeberger, Stefan
author_facet Wolfram, Dolores
Morandi, Evi M.
Eberhart, Nadine
Hautz, Theresa
Hackl, Hubert
Zelger, Bettina
Riede, Gregor
Wachter, Tanja
Dubrac, Sandrine
Ploner, Christian
Pierer, Gerhard
Schneeberger, Stefan
author_sort Wolfram, Dolores
collection PubMed
description Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression still remain a major hurdle for wide adoption of this excellent reconstructive technique. Histopathologic changes during acute skin rejection in vascular composite allotransplantation often mimic inflammatory skin disorders and are hard to distinguish. Hence, the identification of diagnostic and therapeutic markers specific for skin rejection is of particular clinical need. Here we present novel markers allowing for early differentiation between rejection in hind limb allotransplantation and contact hypersensitivity. Assessment of Ccl7, Il18, and Il1b expression is most indicative of distinguishing skin rejection from skin inflammatory disorders. Gene expression levels varied significantly across skin types and regions, indicating localization specific mechanism of leukocyte migration and infiltration. Expression of Il12b, Il17a, and Il1b gene expression levels differed significantly between rejection and inflammation, independent of the skin type. In synopsis of the RNA expression profile and previously assessed protein expression, the Il1 family appears as a promising option for accurate skin rejection diagnosis and, as a following step, for development of novel rejection treatments.
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spelling pubmed-43383832015-03-09 Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis Wolfram, Dolores Morandi, Evi M. Eberhart, Nadine Hautz, Theresa Hackl, Hubert Zelger, Bettina Riede, Gregor Wachter, Tanja Dubrac, Sandrine Ploner, Christian Pierer, Gerhard Schneeberger, Stefan Biomed Res Int Research Article Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression still remain a major hurdle for wide adoption of this excellent reconstructive technique. Histopathologic changes during acute skin rejection in vascular composite allotransplantation often mimic inflammatory skin disorders and are hard to distinguish. Hence, the identification of diagnostic and therapeutic markers specific for skin rejection is of particular clinical need. Here we present novel markers allowing for early differentiation between rejection in hind limb allotransplantation and contact hypersensitivity. Assessment of Ccl7, Il18, and Il1b expression is most indicative of distinguishing skin rejection from skin inflammatory disorders. Gene expression levels varied significantly across skin types and regions, indicating localization specific mechanism of leukocyte migration and infiltration. Expression of Il12b, Il17a, and Il1b gene expression levels differed significantly between rejection and inflammation, independent of the skin type. In synopsis of the RNA expression profile and previously assessed protein expression, the Il1 family appears as a promising option for accurate skin rejection diagnosis and, as a following step, for development of novel rejection treatments. Hindawi Publishing Corporation 2015 2015-02-12 /pmc/articles/PMC4338383/ /pubmed/25756043 http://dx.doi.org/10.1155/2015/259160 Text en Copyright © 2015 Dolores Wolfram et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wolfram, Dolores
Morandi, Evi M.
Eberhart, Nadine
Hautz, Theresa
Hackl, Hubert
Zelger, Bettina
Riede, Gregor
Wachter, Tanja
Dubrac, Sandrine
Ploner, Christian
Pierer, Gerhard
Schneeberger, Stefan
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title_full Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title_fullStr Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title_full_unstemmed Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title_short Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
title_sort differentiation between acute skin rejection in allotransplantation and t-cell mediated skin inflammation based on gene expression analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338383/
https://www.ncbi.nlm.nih.gov/pubmed/25756043
http://dx.doi.org/10.1155/2015/259160
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