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Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats

BACKGROUND: Anti-mullerian hormone (AMH) levels reflect the number of small antral follicles in ovaries and expression changes of AMH and its receptor are suspected to be involved in the pathogenesis of polycystic ovary syndrome (PCOS). OBJECTIVES: The aim of this study was to evaluate gene expressi...

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Autores principales: Daneshian, Zahra, Ramezani Tehrani, Fahimeh, Zarkesh, Maryam, Norooz Zadeh, Mahsa, Mahdian, Reza, Zadeh Vakili, Azita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338645/
https://www.ncbi.nlm.nih.gov/pubmed/25745494
http://dx.doi.org/10.5812/ijem.19511
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author Daneshian, Zahra
Ramezani Tehrani, Fahimeh
Zarkesh, Maryam
Norooz Zadeh, Mahsa
Mahdian, Reza
Zadeh Vakili, Azita
author_facet Daneshian, Zahra
Ramezani Tehrani, Fahimeh
Zarkesh, Maryam
Norooz Zadeh, Mahsa
Mahdian, Reza
Zadeh Vakili, Azita
author_sort Daneshian, Zahra
collection PubMed
description BACKGROUND: Anti-mullerian hormone (AMH) levels reflect the number of small antral follicles in ovaries and expression changes of AMH and its receptor are suspected to be involved in the pathogenesis of polycystic ovary syndrome (PCOS). OBJECTIVES: The aim of this study was to evaluate gene expression of AMH and its receptor in immature and adult rats prenatally exposed to androgen excess. MATERIALS AND METHODS: Six pregnant Wistar rats in the experimental group were treated by subcutaneous injection of 5 mg free testosterone on day 20 of pregnancy, while controls (n = 6) received only 500 mL of solvent. Female pups of each mother were randomly divided into three groups as day 0 (newborn), 10-day old and days 75-85 (adult). RNAs were extracted from ovarian tissues and relative expression levels for AMH and its receptor genes were measured using TaqMan Real-Time PCR. Serum AMH and testosterone levels were measured using ELISA method. RESULTS: Relative AMH expression decreased in newborns, 10-day olds and adults (0.806, 0.443 and 0.809 fold, respectively). AMHR expression was higher in newborns and adults (1.432 and 1.057 fold, respectively), while it decreased by 0.263 fold in 10-day olds, although none of them were significant (P > 0.05). In addition, AMH levels were consistent with the results of gene expression. Testosterone hormone levels from 10 day-olds to adults were significantly increased in both study groups (P = 0.016). CONCLUSIONS: While AMH receptor expression was higher in experimental rats, their serum concentrations of AMH were decreased. Further researches with greater sample sizes and measurement of bioactive forms of hormones are recommended to confirm the findings of this study.
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spelling pubmed-43386452015-03-05 Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats Daneshian, Zahra Ramezani Tehrani, Fahimeh Zarkesh, Maryam Norooz Zadeh, Mahsa Mahdian, Reza Zadeh Vakili, Azita Int J Endocrinol Metab Research Article BACKGROUND: Anti-mullerian hormone (AMH) levels reflect the number of small antral follicles in ovaries and expression changes of AMH and its receptor are suspected to be involved in the pathogenesis of polycystic ovary syndrome (PCOS). OBJECTIVES: The aim of this study was to evaluate gene expression of AMH and its receptor in immature and adult rats prenatally exposed to androgen excess. MATERIALS AND METHODS: Six pregnant Wistar rats in the experimental group were treated by subcutaneous injection of 5 mg free testosterone on day 20 of pregnancy, while controls (n = 6) received only 500 mL of solvent. Female pups of each mother were randomly divided into three groups as day 0 (newborn), 10-day old and days 75-85 (adult). RNAs were extracted from ovarian tissues and relative expression levels for AMH and its receptor genes were measured using TaqMan Real-Time PCR. Serum AMH and testosterone levels were measured using ELISA method. RESULTS: Relative AMH expression decreased in newborns, 10-day olds and adults (0.806, 0.443 and 0.809 fold, respectively). AMHR expression was higher in newborns and adults (1.432 and 1.057 fold, respectively), while it decreased by 0.263 fold in 10-day olds, although none of them were significant (P > 0.05). In addition, AMH levels were consistent with the results of gene expression. Testosterone hormone levels from 10 day-olds to adults were significantly increased in both study groups (P = 0.016). CONCLUSIONS: While AMH receptor expression was higher in experimental rats, their serum concentrations of AMH were decreased. Further researches with greater sample sizes and measurement of bioactive forms of hormones are recommended to confirm the findings of this study. Kowsar 2015-01-30 /pmc/articles/PMC4338645/ /pubmed/25745494 http://dx.doi.org/10.5812/ijem.19511 Text en Copyright © 2015, Research Institute For Endocrine Sciences and Iran Endocrine Society. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Daneshian, Zahra
Ramezani Tehrani, Fahimeh
Zarkesh, Maryam
Norooz Zadeh, Mahsa
Mahdian, Reza
Zadeh Vakili, Azita
Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title_full Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title_fullStr Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title_full_unstemmed Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title_short Antimullerian Hormone and Its Receptor Gene Expression in Prenatally Androgenized Female Rats
title_sort antimullerian hormone and its receptor gene expression in prenatally androgenized female rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338645/
https://www.ncbi.nlm.nih.gov/pubmed/25745494
http://dx.doi.org/10.5812/ijem.19511
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