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Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling

During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain fill...

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Autores principales: Kohl, Stefan, Hollmann, Julien, Erban, Alexander, Kopka, Joachim, Riewe, David, Weschke, Winfriede, Weber, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339599/
https://www.ncbi.nlm.nih.gov/pubmed/25617470
http://dx.doi.org/10.1093/jxb/eru492
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author Kohl, Stefan
Hollmann, Julien
Erban, Alexander
Kopka, Joachim
Riewe, David
Weschke, Winfriede
Weber, Hans
author_facet Kohl, Stefan
Hollmann, Julien
Erban, Alexander
Kopka, Joachim
Riewe, David
Weschke, Winfriede
Weber, Hans
author_sort Kohl, Stefan
collection PubMed
description During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel transcript and metabolite profiling in glumes and endosperm were performed, showing that glume metabolism and development adjusts to changing grain demands, reflected by specific signatures of metabolite and transcript abundances. Before high endosperm sink strength is established by storage product accumulation, glumes form early, intermediary sink organs, shifting then to remobilizing and exporting source organs. Metabolic and transcriptional transitions occur at two phases: first, at the onset of endosperm filling, as a consequence of endosperm sink activity and assimilate depletion in endosperm and vascular tissues; second, at late grain filling, by developmental ageing and senescence. Regulation of and transition between phases are probably governed by specific NAC and WRKY transcription factors, and both abscisic and jasmonic acid, and are accompanied by changed expression of specific nitrogen transporters. Expression and metabolite profiling suggest glume-specific mechanisms of assimilate conversion and translocation. In summary, grain filling and endosperm sink strength coordinate phase changes in glumes via metabolic, hormonal, and transcriptional control. This study provides a comprehensive view of barley glume development and metabolism, and identifies candidate genes and associated pathways, potentially important for breeding improved grain traits.
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spelling pubmed-43395992015-03-18 Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling Kohl, Stefan Hollmann, Julien Erban, Alexander Kopka, Joachim Riewe, David Weschke, Winfriede Weber, Hans J Exp Bot Research Paper During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel transcript and metabolite profiling in glumes and endosperm were performed, showing that glume metabolism and development adjusts to changing grain demands, reflected by specific signatures of metabolite and transcript abundances. Before high endosperm sink strength is established by storage product accumulation, glumes form early, intermediary sink organs, shifting then to remobilizing and exporting source organs. Metabolic and transcriptional transitions occur at two phases: first, at the onset of endosperm filling, as a consequence of endosperm sink activity and assimilate depletion in endosperm and vascular tissues; second, at late grain filling, by developmental ageing and senescence. Regulation of and transition between phases are probably governed by specific NAC and WRKY transcription factors, and both abscisic and jasmonic acid, and are accompanied by changed expression of specific nitrogen transporters. Expression and metabolite profiling suggest glume-specific mechanisms of assimilate conversion and translocation. In summary, grain filling and endosperm sink strength coordinate phase changes in glumes via metabolic, hormonal, and transcriptional control. This study provides a comprehensive view of barley glume development and metabolism, and identifies candidate genes and associated pathways, potentially important for breeding improved grain traits. Oxford University Press 2015-03 2015-01-22 /pmc/articles/PMC4339599/ /pubmed/25617470 http://dx.doi.org/10.1093/jxb/eru492 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Kohl, Stefan
Hollmann, Julien
Erban, Alexander
Kopka, Joachim
Riewe, David
Weschke, Winfriede
Weber, Hans
Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title_full Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title_fullStr Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title_full_unstemmed Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title_short Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
title_sort metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339599/
https://www.ncbi.nlm.nih.gov/pubmed/25617470
http://dx.doi.org/10.1093/jxb/eru492
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