Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆

In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson's Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify...

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Autores principales: Tang, Yue, Cui, Yongchun, Luo, Fuliang, Liu, Xiaopeng, Wang, Xiaojuan, Wu, Aili, Zhao, Junwei, Tian, Zhong, Wu, Like
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Neurodegenerative Disease and Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340024/
https://www.ncbi.nlm.nih.gov/pubmed/25722701
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.14.009
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author Tang, Yue
Cui, Yongchun
Luo, Fuliang
Liu, Xiaopeng
Wang, Xiaojuan
Wu, Aili
Zhao, Junwei
Tian, Zhong
Wu, Like
author_facet Tang, Yue
Cui, Yongchun
Luo, Fuliang
Liu, Xiaopeng
Wang, Xiaojuan
Wu, Aili
Zhao, Junwei
Tian, Zhong
Wu, Like
author_sort Tang, Yue
collection PubMed
description In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson's Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal α-synuclein accumulation in cells. Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells.
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spelling pubmed-43400242015-02-26 Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆ Tang, Yue Cui, Yongchun Luo, Fuliang Liu, Xiaopeng Wang, Xiaojuan Wu, Aili Zhao, Junwei Tian, Zhong Wu, Like Neural Regen Res Research and Report: Neurodegenerative Disease and Neural Regeneration In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson's Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal α-synuclein accumulation in cells. Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells. Medknow Publications & Media Pvt Ltd 2012-05-15 /pmc/articles/PMC4340024/ /pubmed/25722701 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.14.009 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Neurodegenerative Disease and Neural Regeneration
Tang, Yue
Cui, Yongchun
Luo, Fuliang
Liu, Xiaopeng
Wang, Xiaojuan
Wu, Aili
Zhao, Junwei
Tian, Zhong
Wu, Like
Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title_full Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title_fullStr Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title_full_unstemmed Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title_short Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
title_sort cell viability and dopamine secretion of 6-hydroxydopamine-treated pc12 cells co-cultured with bone marrow-derived mesenchymal stem cells☆
topic Research and Report: Neurodegenerative Disease and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340024/
https://www.ncbi.nlm.nih.gov/pubmed/25722701
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.14.009
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