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Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation
In this study, Schwann cells, at a density of 1 × 10(5) cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. Schwann cells cultured on the silk fibroin nanofibers appeared more ordered, their processes extended further, and they fo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340035/ https://www.ncbi.nlm.nih.gov/pubmed/25722711 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.15.008 |
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author | Hu, Aijun Zuo, Baoqi Zhang, Feng Lan, Qing Zhang, Huanxiang |
author_facet | Hu, Aijun Zuo, Baoqi Zhang, Feng Lan, Qing Zhang, Huanxiang |
author_sort | Hu, Aijun |
collection | PubMed |
description | In this study, Schwann cells, at a density of 1 × 10(5) cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. Schwann cells cultured on the silk fibroin nanofibers appeared more ordered, their processes extended further, and they formed more extensive and complex interconnections. In addition, the silk fibroin nanofibers had no impact on the proliferation of Schwann cells or on the secretion of ciliary neurotrophic factor, brain-derived neurotrophic factor or nerve growth factor. These findings indicate that regenerated electrospun silk fibroin nanofibers can promote Schwann cell adhesion, growth and proliferation, and have excellent biocompatibility. |
format | Online Article Text |
id | pubmed-4340035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43400352015-02-26 Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation Hu, Aijun Zuo, Baoqi Zhang, Feng Lan, Qing Zhang, Huanxiang Neural Regen Res Techniques and Methods: Emerging Technology in Neural Regeneration In this study, Schwann cells, at a density of 1 × 10(5) cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. Schwann cells cultured on the silk fibroin nanofibers appeared more ordered, their processes extended further, and they formed more extensive and complex interconnections. In addition, the silk fibroin nanofibers had no impact on the proliferation of Schwann cells or on the secretion of ciliary neurotrophic factor, brain-derived neurotrophic factor or nerve growth factor. These findings indicate that regenerated electrospun silk fibroin nanofibers can promote Schwann cell adhesion, growth and proliferation, and have excellent biocompatibility. Medknow Publications & Media Pvt Ltd 2012-05-25 /pmc/articles/PMC4340035/ /pubmed/25722711 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.15.008 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Techniques and Methods: Emerging Technology in Neural Regeneration Hu, Aijun Zuo, Baoqi Zhang, Feng Lan, Qing Zhang, Huanxiang Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title_full | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title_fullStr | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title_full_unstemmed | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title_short | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation |
title_sort | electrospun silk fibroin nanofibers promote schwann cell adhesion, growth and proliferation |
topic | Techniques and Methods: Emerging Technology in Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340035/ https://www.ncbi.nlm.nih.gov/pubmed/25722711 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.15.008 |
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