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Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein
Ectotherms in northern latitudes are seasonally exposed to cold temperatures. To improve survival under cold stress, they use diverse mechanisms to increase temperature resistance and prevent tissue damage. The accumulation of anti-freeze proteins that improve cold hardiness occurs in diverse specie...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340617/ https://www.ncbi.nlm.nih.gov/pubmed/25714402 http://dx.doi.org/10.1371/journal.pone.0116562 |
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author | Heisig, Martin Mattessich, Sarah Rembisz, Alison Acar, Ali Shapiro, Martin Booth, Carmen J. Neelakanta, Girish Fikrig, Erol |
author_facet | Heisig, Martin Mattessich, Sarah Rembisz, Alison Acar, Ali Shapiro, Martin Booth, Carmen J. Neelakanta, Girish Fikrig, Erol |
author_sort | Heisig, Martin |
collection | PubMed |
description | Ectotherms in northern latitudes are seasonally exposed to cold temperatures. To improve survival under cold stress, they use diverse mechanisms to increase temperature resistance and prevent tissue damage. The accumulation of anti-freeze proteins that improve cold hardiness occurs in diverse species including plants, arthropods, fish, and amphibians. We previously identified an Ixodes scapularis anti-freeze glycoprotein, named IAFGP, and demonstrated its cold protective function in the natural tick host and in a transgenic Drosophila model. Here we show, in a transgenic mouse model expressing an anti-freeze glycoprotein, that IAFGP protects mammalian cells and mice from cold shock and frostbite respectively. Transgenic skin samples showed reduced cell death upon cold storage ex vivo and transgenic mice demonstrated increased resistance to frostbite injury in vivo. IAFGP actively protects mammalian tissue from freezing, suggesting its application for the prevention of frostbite, and other diseases associated with cold exposure. |
format | Online Article Text |
id | pubmed-4340617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43406172015-03-04 Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein Heisig, Martin Mattessich, Sarah Rembisz, Alison Acar, Ali Shapiro, Martin Booth, Carmen J. Neelakanta, Girish Fikrig, Erol PLoS One Research Article Ectotherms in northern latitudes are seasonally exposed to cold temperatures. To improve survival under cold stress, they use diverse mechanisms to increase temperature resistance and prevent tissue damage. The accumulation of anti-freeze proteins that improve cold hardiness occurs in diverse species including plants, arthropods, fish, and amphibians. We previously identified an Ixodes scapularis anti-freeze glycoprotein, named IAFGP, and demonstrated its cold protective function in the natural tick host and in a transgenic Drosophila model. Here we show, in a transgenic mouse model expressing an anti-freeze glycoprotein, that IAFGP protects mammalian cells and mice from cold shock and frostbite respectively. Transgenic skin samples showed reduced cell death upon cold storage ex vivo and transgenic mice demonstrated increased resistance to frostbite injury in vivo. IAFGP actively protects mammalian tissue from freezing, suggesting its application for the prevention of frostbite, and other diseases associated with cold exposure. Public Library of Science 2015-02-25 /pmc/articles/PMC4340617/ /pubmed/25714402 http://dx.doi.org/10.1371/journal.pone.0116562 Text en © 2015 Heisig et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Heisig, Martin Mattessich, Sarah Rembisz, Alison Acar, Ali Shapiro, Martin Booth, Carmen J. Neelakanta, Girish Fikrig, Erol Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title | Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title_full | Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title_fullStr | Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title_full_unstemmed | Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title_short | Frostbite Protection in Mice Expressing an Antifreeze Glycoprotein |
title_sort | frostbite protection in mice expressing an antifreeze glycoprotein |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340617/ https://www.ncbi.nlm.nih.gov/pubmed/25714402 http://dx.doi.org/10.1371/journal.pone.0116562 |
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