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IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation
BACKGROUND: Interleukin-27 (IL-27) is a multifunctional cytokine with both pro-inflammatory and immunoregulatory functions. At present, the role of IL-27 in pulmonary fibrosis remains unknown. METHODS: In this study, we observed the expression of IL-27/IL-27R in a mouse model of bleomycin (BLM)-indu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340860/ https://www.ncbi.nlm.nih.gov/pubmed/25888222 http://dx.doi.org/10.1186/s12890-015-0012-4 |
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author | Dong, Zhaoxing Lu, Xin Yang, Yanni Zhang, Tao Li, Yongxia Chai, Yanlin Lei, Wen Li, Changbo Ai, Li Tai, Wenlin |
author_facet | Dong, Zhaoxing Lu, Xin Yang, Yanni Zhang, Tao Li, Yongxia Chai, Yanlin Lei, Wen Li, Changbo Ai, Li Tai, Wenlin |
author_sort | Dong, Zhaoxing |
collection | PubMed |
description | BACKGROUND: Interleukin-27 (IL-27) is a multifunctional cytokine with both pro-inflammatory and immunoregulatory functions. At present, the role of IL-27 in pulmonary fibrosis remains unknown. METHODS: In this study, we observed the expression of IL-27/IL-27R in a mouse model of bleomycin (BLM)-induced pulmonary fibrosis. We verified the role of IL-27 using hematoxylin and eosin as well as Masson’s staining methods and measuring the content of hydroxyproline as well as collagen I and III. We assessed the differentiation of T lymphocytes in the spleen and measured the concentration of cytokines in bronchoalveolar lavage fluid (BALF) and the expression level of relevant proteins in the JAK/STAT and TGF-ß/Smad signaling pathways in lung tissue. RESULTS: Increased IL-27 expression in BLM-induced pulmonary fibrosis was noted. IL-27 treatment may alleviate pulmonary fibrosis and increase the survival of mice. IL-27 inhibited the development of CD4(+) IL-17(+), CD4(+) IL-4(+) T, and CD4(+) Foxp3(+) cells and the secretion of IL-17, IL-4, IL-6, and TGF-ß. IL-27 induced the production of CD4(+) IL-10(+) and CD4(+) INF-γ(+) T cells. IL-27 decreased the levels of phosphorylated STAT1, STAT3, STAT5, Smad1, and Smad3 but increased the level of SOCS3. CONCLUSIONS: This study demonstrates that IL-27 potentially attenuates BLM-induced pulmonary fibrosis by regulating Th17 differentiation and cytokine secretion. |
format | Online Article Text |
id | pubmed-4340860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43408602015-02-27 IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation Dong, Zhaoxing Lu, Xin Yang, Yanni Zhang, Tao Li, Yongxia Chai, Yanlin Lei, Wen Li, Changbo Ai, Li Tai, Wenlin BMC Pulm Med Research Article BACKGROUND: Interleukin-27 (IL-27) is a multifunctional cytokine with both pro-inflammatory and immunoregulatory functions. At present, the role of IL-27 in pulmonary fibrosis remains unknown. METHODS: In this study, we observed the expression of IL-27/IL-27R in a mouse model of bleomycin (BLM)-induced pulmonary fibrosis. We verified the role of IL-27 using hematoxylin and eosin as well as Masson’s staining methods and measuring the content of hydroxyproline as well as collagen I and III. We assessed the differentiation of T lymphocytes in the spleen and measured the concentration of cytokines in bronchoalveolar lavage fluid (BALF) and the expression level of relevant proteins in the JAK/STAT and TGF-ß/Smad signaling pathways in lung tissue. RESULTS: Increased IL-27 expression in BLM-induced pulmonary fibrosis was noted. IL-27 treatment may alleviate pulmonary fibrosis and increase the survival of mice. IL-27 inhibited the development of CD4(+) IL-17(+), CD4(+) IL-4(+) T, and CD4(+) Foxp3(+) cells and the secretion of IL-17, IL-4, IL-6, and TGF-ß. IL-27 induced the production of CD4(+) IL-10(+) and CD4(+) INF-γ(+) T cells. IL-27 decreased the levels of phosphorylated STAT1, STAT3, STAT5, Smad1, and Smad3 but increased the level of SOCS3. CONCLUSIONS: This study demonstrates that IL-27 potentially attenuates BLM-induced pulmonary fibrosis by regulating Th17 differentiation and cytokine secretion. BioMed Central 2015-02-18 /pmc/articles/PMC4340860/ /pubmed/25888222 http://dx.doi.org/10.1186/s12890-015-0012-4 Text en © Dong et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Dong, Zhaoxing Lu, Xin Yang, Yanni Zhang, Tao Li, Yongxia Chai, Yanlin Lei, Wen Li, Changbo Ai, Li Tai, Wenlin IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title | IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title_full | IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title_fullStr | IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title_full_unstemmed | IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title_short | IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation |
title_sort | il-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the th17 cell differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340860/ https://www.ncbi.nlm.nih.gov/pubmed/25888222 http://dx.doi.org/10.1186/s12890-015-0012-4 |
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