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Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size

OBJECTIVE(S): Previous studies demonstrate that changes in pre-mRNA splicing play a significant role in human disease development. Furthermore, many cancer-associated genes are regulated by alternative splicing. There are mounting evidences that splice variants which express predominantly in tumors,...

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Autores principales: Rahmati, Shima, Emadi-Baygi, Modjtaba, Nikpour, Parvaneh, Emadi-Andani, Elaheh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340984/
https://www.ncbi.nlm.nih.gov/pubmed/25729545
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author Rahmati, Shima
Emadi-Baygi, Modjtaba
Nikpour, Parvaneh
Emadi-Andani, Elaheh
author_facet Rahmati, Shima
Emadi-Baygi, Modjtaba
Nikpour, Parvaneh
Emadi-Andani, Elaheh
author_sort Rahmati, Shima
collection PubMed
description OBJECTIVE(S): Previous studies demonstrate that changes in pre-mRNA splicing play a significant role in human disease development. Furthermore, many cancer-associated genes are regulated by alternative splicing. There are mounting evidences that splice variants which express predominantly in tumors, have clear diagnostic value and may provide potential drug targets. Located on the X chromosome, ZFX gene functions as a transcription regulator for self-renewal of stem cells. This gene has 5 splice variants that encode 3 isoforms. In the present study, we evaluated the clinicopathological relevance of the expression of ZFX isoform 3/variant 5 gene in gastric carcinoma. MATERIALS AND METHODS: A total of 60 tumoral and non-tumoral gastric specimens were evaluated for ZFX isoform 3/variant 5 gene expression using quantitative real-time PCR. RESULTS: Our results showed that the expression of ZFX isoform 3/variant 5 transcript was heterogeneous in gastric specimens. We further showed that there was a positive correlation between the variant expression and tumor size, but not with other clinicopathological features of gastric tumors. CONCLUSION: This report shows that the expression of ZFX isoform 3/variant 5 transcript was heterogeneous in gastric specimens. Furthermore, there was no significant association between ZFX isoform 3/variant 5 expression and most of clinicopathological features of gastric tumors except for a positive correlation with tumor size. The elucidation of the precise molecular mechanisms governed by the ZFX isoforms/variants needs further investigation.
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spelling pubmed-43409842015-02-27 Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size Rahmati, Shima Emadi-Baygi, Modjtaba Nikpour, Parvaneh Emadi-Andani, Elaheh Iran J Basic Med Sci Original Article OBJECTIVE(S): Previous studies demonstrate that changes in pre-mRNA splicing play a significant role in human disease development. Furthermore, many cancer-associated genes are regulated by alternative splicing. There are mounting evidences that splice variants which express predominantly in tumors, have clear diagnostic value and may provide potential drug targets. Located on the X chromosome, ZFX gene functions as a transcription regulator for self-renewal of stem cells. This gene has 5 splice variants that encode 3 isoforms. In the present study, we evaluated the clinicopathological relevance of the expression of ZFX isoform 3/variant 5 gene in gastric carcinoma. MATERIALS AND METHODS: A total of 60 tumoral and non-tumoral gastric specimens were evaluated for ZFX isoform 3/variant 5 gene expression using quantitative real-time PCR. RESULTS: Our results showed that the expression of ZFX isoform 3/variant 5 transcript was heterogeneous in gastric specimens. We further showed that there was a positive correlation between the variant expression and tumor size, but not with other clinicopathological features of gastric tumors. CONCLUSION: This report shows that the expression of ZFX isoform 3/variant 5 transcript was heterogeneous in gastric specimens. Furthermore, there was no significant association between ZFX isoform 3/variant 5 expression and most of clinicopathological features of gastric tumors except for a positive correlation with tumor size. The elucidation of the precise molecular mechanisms governed by the ZFX isoforms/variants needs further investigation. Mashhad University of Medical Sciences 2014-10 /pmc/articles/PMC4340984/ /pubmed/25729545 Text en © Iranian Journal of Basic Medical Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rahmati, Shima
Emadi-Baygi, Modjtaba
Nikpour, Parvaneh
Emadi-Andani, Elaheh
Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title_full Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title_fullStr Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title_full_unstemmed Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title_short Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size
title_sort expression profile of zfx isoform3/variant 5 in gastric cancer tissues and its association with tumor size
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340984/
https://www.ncbi.nlm.nih.gov/pubmed/25729545
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