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A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks

Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circ...

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Autores principales: Erbs, Eric, Faget, Lauren, Scherrer, Gregory, Matifas, Audrey, Filliol, Dominique, Vonesch, Jean-Luc, Koch, Marc, Kessler, Pascal, Hentsch, Didier, Birling, Marie-Christine, Koutsourakis, Manoussos, Vasseur, Laurent, Veinante, Pierre, Kieffer, Brigitte L., Massotte, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341027/
https://www.ncbi.nlm.nih.gov/pubmed/24623156
http://dx.doi.org/10.1007/s00429-014-0717-9
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author Erbs, Eric
Faget, Lauren
Scherrer, Gregory
Matifas, Audrey
Filliol, Dominique
Vonesch, Jean-Luc
Koch, Marc
Kessler, Pascal
Hentsch, Didier
Birling, Marie-Christine
Koutsourakis, Manoussos
Vasseur, Laurent
Veinante, Pierre
Kieffer, Brigitte L.
Massotte, Dominique
author_facet Erbs, Eric
Faget, Lauren
Scherrer, Gregory
Matifas, Audrey
Filliol, Dominique
Vonesch, Jean-Luc
Koch, Marc
Kessler, Pascal
Hentsch, Didier
Birling, Marie-Christine
Koutsourakis, Manoussos
Vasseur, Laurent
Veinante, Pierre
Kieffer, Brigitte L.
Massotte, Dominique
author_sort Erbs, Eric
collection PubMed
description Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circuitry, cellular or molecular levels remains unsolved. To challenge the hypothesis of MOR/DOR heteromerization in the brain, we generated redMOR/greenDOR double knock-in mice and report dual receptor mapping throughout the nervous system. Data are organized as an interactive database offering an opioid receptor atlas with concomitant MOR/DOR visualization at subcellular resolution, accessible online. We also provide co-immunoprecipitation-based evidence for receptor heteromerization in these mice. In the forebrain, MOR and DOR are mainly detected in separate neurons, suggesting system-level interactions in high-order processing. In contrast, neuronal co-localization is detected in subcortical networks essential for survival involved in eating and sexual behaviors or perception and response to aversive stimuli. In addition, potential MOR/DOR intracellular interactions within the nociceptive pathway offer novel therapeutic perspectives. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-014-0717-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-43410272015-03-03 A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks Erbs, Eric Faget, Lauren Scherrer, Gregory Matifas, Audrey Filliol, Dominique Vonesch, Jean-Luc Koch, Marc Kessler, Pascal Hentsch, Didier Birling, Marie-Christine Koutsourakis, Manoussos Vasseur, Laurent Veinante, Pierre Kieffer, Brigitte L. Massotte, Dominique Brain Struct Funct Original Article Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circuitry, cellular or molecular levels remains unsolved. To challenge the hypothesis of MOR/DOR heteromerization in the brain, we generated redMOR/greenDOR double knock-in mice and report dual receptor mapping throughout the nervous system. Data are organized as an interactive database offering an opioid receptor atlas with concomitant MOR/DOR visualization at subcellular resolution, accessible online. We also provide co-immunoprecipitation-based evidence for receptor heteromerization in these mice. In the forebrain, MOR and DOR are mainly detected in separate neurons, suggesting system-level interactions in high-order processing. In contrast, neuronal co-localization is detected in subcortical networks essential for survival involved in eating and sexual behaviors or perception and response to aversive stimuli. In addition, potential MOR/DOR intracellular interactions within the nociceptive pathway offer novel therapeutic perspectives. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-014-0717-9) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-03-13 2015 /pmc/articles/PMC4341027/ /pubmed/24623156 http://dx.doi.org/10.1007/s00429-014-0717-9 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Erbs, Eric
Faget, Lauren
Scherrer, Gregory
Matifas, Audrey
Filliol, Dominique
Vonesch, Jean-Luc
Koch, Marc
Kessler, Pascal
Hentsch, Didier
Birling, Marie-Christine
Koutsourakis, Manoussos
Vasseur, Laurent
Veinante, Pierre
Kieffer, Brigitte L.
Massotte, Dominique
A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title_full A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title_fullStr A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title_full_unstemmed A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title_short A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
title_sort mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341027/
https://www.ncbi.nlm.nih.gov/pubmed/24623156
http://dx.doi.org/10.1007/s00429-014-0717-9
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