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A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circ...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341027/ https://www.ncbi.nlm.nih.gov/pubmed/24623156 http://dx.doi.org/10.1007/s00429-014-0717-9 |
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author | Erbs, Eric Faget, Lauren Scherrer, Gregory Matifas, Audrey Filliol, Dominique Vonesch, Jean-Luc Koch, Marc Kessler, Pascal Hentsch, Didier Birling, Marie-Christine Koutsourakis, Manoussos Vasseur, Laurent Veinante, Pierre Kieffer, Brigitte L. Massotte, Dominique |
author_facet | Erbs, Eric Faget, Lauren Scherrer, Gregory Matifas, Audrey Filliol, Dominique Vonesch, Jean-Luc Koch, Marc Kessler, Pascal Hentsch, Didier Birling, Marie-Christine Koutsourakis, Manoussos Vasseur, Laurent Veinante, Pierre Kieffer, Brigitte L. Massotte, Dominique |
author_sort | Erbs, Eric |
collection | PubMed |
description | Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circuitry, cellular or molecular levels remains unsolved. To challenge the hypothesis of MOR/DOR heteromerization in the brain, we generated redMOR/greenDOR double knock-in mice and report dual receptor mapping throughout the nervous system. Data are organized as an interactive database offering an opioid receptor atlas with concomitant MOR/DOR visualization at subcellular resolution, accessible online. We also provide co-immunoprecipitation-based evidence for receptor heteromerization in these mice. In the forebrain, MOR and DOR are mainly detected in separate neurons, suggesting system-level interactions in high-order processing. In contrast, neuronal co-localization is detected in subcortical networks essential for survival involved in eating and sexual behaviors or perception and response to aversive stimuli. In addition, potential MOR/DOR intracellular interactions within the nociceptive pathway offer novel therapeutic perspectives. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-014-0717-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4341027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-43410272015-03-03 A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks Erbs, Eric Faget, Lauren Scherrer, Gregory Matifas, Audrey Filliol, Dominique Vonesch, Jean-Luc Koch, Marc Kessler, Pascal Hentsch, Didier Birling, Marie-Christine Koutsourakis, Manoussos Vasseur, Laurent Veinante, Pierre Kieffer, Brigitte L. Massotte, Dominique Brain Struct Funct Original Article Opioid receptors are G protein-coupled receptors (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) and delta (DOR) opioid receptors functionally interact in vivo, but whether interactions occur at circuitry, cellular or molecular levels remains unsolved. To challenge the hypothesis of MOR/DOR heteromerization in the brain, we generated redMOR/greenDOR double knock-in mice and report dual receptor mapping throughout the nervous system. Data are organized as an interactive database offering an opioid receptor atlas with concomitant MOR/DOR visualization at subcellular resolution, accessible online. We also provide co-immunoprecipitation-based evidence for receptor heteromerization in these mice. In the forebrain, MOR and DOR are mainly detected in separate neurons, suggesting system-level interactions in high-order processing. In contrast, neuronal co-localization is detected in subcortical networks essential for survival involved in eating and sexual behaviors or perception and response to aversive stimuli. In addition, potential MOR/DOR intracellular interactions within the nociceptive pathway offer novel therapeutic perspectives. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-014-0717-9) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-03-13 2015 /pmc/articles/PMC4341027/ /pubmed/24623156 http://dx.doi.org/10.1007/s00429-014-0717-9 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Article Erbs, Eric Faget, Lauren Scherrer, Gregory Matifas, Audrey Filliol, Dominique Vonesch, Jean-Luc Koch, Marc Kessler, Pascal Hentsch, Didier Birling, Marie-Christine Koutsourakis, Manoussos Vasseur, Laurent Veinante, Pierre Kieffer, Brigitte L. Massotte, Dominique A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title | A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title_full | A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title_fullStr | A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title_full_unstemmed | A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title_short | A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
title_sort | mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341027/ https://www.ncbi.nlm.nih.gov/pubmed/24623156 http://dx.doi.org/10.1007/s00429-014-0717-9 |
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