The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line

Despite its claimed therapeutic effects, the action of sea cucumber (known as gamat in the Malay language) on human osteoblast cells is still unknown. We performed in vitro studies utilising extract of Stichopus sp1 (gamat) to elucidate its effects on cell viability and functional activity. We found...

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Autores principales: Shahrulazua, A, Samsudin, AR, Iskandar, MA, Amran, AS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Malaysian Orthopaedic Association 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341048/
https://www.ncbi.nlm.nih.gov/pubmed/25722806
http://dx.doi.org/10.5704/MOJ.1303.015
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author Shahrulazua, A
Samsudin, AR
Iskandar, MA
Amran, AS
author_facet Shahrulazua, A
Samsudin, AR
Iskandar, MA
Amran, AS
author_sort Shahrulazua, A
collection PubMed
description Despite its claimed therapeutic effects, the action of sea cucumber (known as gamat in the Malay language) on human osteoblast cells is still unknown. We performed in vitro studies utilising extract of Stichopus sp1 (gamat) to elucidate its effects on cell viability and functional activity. We found an inverse relationship between gamat concentration and its effect on osteoblast cell viability (p<0.001). Only gamat concentration at 1mg/ml significantly promoted cell viability at day 3 of incubation. There was a trend towards increased osteoblast cell function in the presence of gamat at 5mg/ml and 10mg/ml but this observation was not consistent at different incubation periods. KEY WORDS: Alkaline phosphatase, Stichopus, Sea Cucumbers, Osteoblasts, Tetrazolium Salts
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spelling pubmed-43410482015-02-26 The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line Shahrulazua, A Samsudin, AR Iskandar, MA Amran, AS Malays Orthop J Research Article Despite its claimed therapeutic effects, the action of sea cucumber (known as gamat in the Malay language) on human osteoblast cells is still unknown. We performed in vitro studies utilising extract of Stichopus sp1 (gamat) to elucidate its effects on cell viability and functional activity. We found an inverse relationship between gamat concentration and its effect on osteoblast cell viability (p<0.001). Only gamat concentration at 1mg/ml significantly promoted cell viability at day 3 of incubation. There was a trend towards increased osteoblast cell function in the presence of gamat at 5mg/ml and 10mg/ml but this observation was not consistent at different incubation periods. KEY WORDS: Alkaline phosphatase, Stichopus, Sea Cucumbers, Osteoblasts, Tetrazolium Salts Malaysian Orthopaedic Association 2013-03 /pmc/articles/PMC4341048/ /pubmed/25722806 http://dx.doi.org/10.5704/MOJ.1303.015 Text en Copyright © 2014, Malaysian Orthopaedic Journal This article is distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Article
Shahrulazua, A
Samsudin, AR
Iskandar, MA
Amran, AS
The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title_full The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title_fullStr The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title_full_unstemmed The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title_short The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line
title_sort in-vitro effects of sea cucumber (stichopus sp1) extract on human osteoblast cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341048/
https://www.ncbi.nlm.nih.gov/pubmed/25722806
http://dx.doi.org/10.5704/MOJ.1303.015
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