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PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy
During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to prov...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341069/ https://www.ncbi.nlm.nih.gov/pubmed/25362091 http://dx.doi.org/10.1093/bioinformatics/btu720 |
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author | Pengo, Thomas Holden, Seamus J. Manley, Suliana |
author_facet | Pengo, Thomas Holden, Seamus J. Manley, Suliana |
author_sort | Pengo, Thomas |
collection | PubMed |
description | During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software. Contact: thomas.pengo@gmail.com |
format | Online Article Text |
id | pubmed-4341069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43410692015-03-10 PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy Pengo, Thomas Holden, Seamus J. Manley, Suliana Bioinformatics Applications Notes During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software. Contact: thomas.pengo@gmail.com Oxford University Press 2015-03-01 2014-10-31 /pmc/articles/PMC4341069/ /pubmed/25362091 http://dx.doi.org/10.1093/bioinformatics/btu720 Text en © The Author 2014. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Applications Notes Pengo, Thomas Holden, Seamus J. Manley, Suliana PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title | PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title_full | PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title_fullStr | PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title_full_unstemmed | PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title_short | PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy |
title_sort | palmsiever: a tool to turn raw data into results for single-molecule localization microscopy |
topic | Applications Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341069/ https://www.ncbi.nlm.nih.gov/pubmed/25362091 http://dx.doi.org/10.1093/bioinformatics/btu720 |
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