Sub-100 nm resolution microscopy based on proximity projection grating scheme

Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured illumination microscopy using the proximity projecti...

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Detalles Bibliográficos
Autores principales: Hu, Feng, Somekh, Michael G., Albutt, Darren J., Webb, Kevin, Moradi, Emilia, See, Chung W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341199/
https://www.ncbi.nlm.nih.gov/pubmed/25715953
http://dx.doi.org/10.1038/srep08589
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author Hu, Feng
Somekh, Michael G.
Albutt, Darren J.
Webb, Kevin
Moradi, Emilia
See, Chung W.
author_facet Hu, Feng
Somekh, Michael G.
Albutt, Darren J.
Webb, Kevin
Moradi, Emilia
See, Chung W.
author_sort Hu, Feng
collection PubMed
description Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured illumination microscopy using the proximity projection grating scheme (PPGS), which has the ability to further enhance the SIM resolution without invoking any nonlinearity response from the sample. With the PPGS-based SIM, sub-100 nm resolution has been obtained experimentally, and results corresponding to 2.4 times resolution improvement are presented. Furthermore, it will be shown that an improvement of greater than 3 times can be achieved.
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spelling pubmed-43411992015-03-04 Sub-100 nm resolution microscopy based on proximity projection grating scheme Hu, Feng Somekh, Michael G. Albutt, Darren J. Webb, Kevin Moradi, Emilia See, Chung W. Sci Rep Article Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured illumination microscopy using the proximity projection grating scheme (PPGS), which has the ability to further enhance the SIM resolution without invoking any nonlinearity response from the sample. With the PPGS-based SIM, sub-100 nm resolution has been obtained experimentally, and results corresponding to 2.4 times resolution improvement are presented. Furthermore, it will be shown that an improvement of greater than 3 times can be achieved. Nature Publishing Group 2015-02-26 /pmc/articles/PMC4341199/ /pubmed/25715953 http://dx.doi.org/10.1038/srep08589 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hu, Feng
Somekh, Michael G.
Albutt, Darren J.
Webb, Kevin
Moradi, Emilia
See, Chung W.
Sub-100 nm resolution microscopy based on proximity projection grating scheme
title Sub-100 nm resolution microscopy based on proximity projection grating scheme
title_full Sub-100 nm resolution microscopy based on proximity projection grating scheme
title_fullStr Sub-100 nm resolution microscopy based on proximity projection grating scheme
title_full_unstemmed Sub-100 nm resolution microscopy based on proximity projection grating scheme
title_short Sub-100 nm resolution microscopy based on proximity projection grating scheme
title_sort sub-100 nm resolution microscopy based on proximity projection grating scheme
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341199/
https://www.ncbi.nlm.nih.gov/pubmed/25715953
http://dx.doi.org/10.1038/srep08589
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