Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking

We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both...

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Autores principales: Yapici, Nazmiye B., Bi, Yue, Li, Pengfei, Chen, Xin, Yan, Xin, Mandalapu, Srinivas Rao, Faucett, Megan, Jockusch, Steffen, Ju, Jingfang, Gibson, K. Michael, Pavan, William J., Bi, Lanrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341211/
https://www.ncbi.nlm.nih.gov/pubmed/25715948
http://dx.doi.org/10.1038/srep08576
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author Yapici, Nazmiye B.
Bi, Yue
Li, Pengfei
Chen, Xin
Yan, Xin
Mandalapu, Srinivas Rao
Faucett, Megan
Jockusch, Steffen
Ju, Jingfang
Gibson, K. Michael
Pavan, William J.
Bi, Lanrong
author_facet Yapici, Nazmiye B.
Bi, Yue
Li, Pengfei
Chen, Xin
Yan, Xin
Mandalapu, Srinivas Rao
Faucett, Megan
Jockusch, Steffen
Ju, Jingfang
Gibson, K. Michael
Pavan, William J.
Bi, Lanrong
author_sort Yapici, Nazmiye B.
collection PubMed
description We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis.
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spelling pubmed-43412112015-03-04 Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking Yapici, Nazmiye B. Bi, Yue Li, Pengfei Chen, Xin Yan, Xin Mandalapu, Srinivas Rao Faucett, Megan Jockusch, Steffen Ju, Jingfang Gibson, K. Michael Pavan, William J. Bi, Lanrong Sci Rep Article We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis. Nature Publishing Group 2015-02-26 /pmc/articles/PMC4341211/ /pubmed/25715948 http://dx.doi.org/10.1038/srep08576 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yapici, Nazmiye B.
Bi, Yue
Li, Pengfei
Chen, Xin
Yan, Xin
Mandalapu, Srinivas Rao
Faucett, Megan
Jockusch, Steffen
Ju, Jingfang
Gibson, K. Michael
Pavan, William J.
Bi, Lanrong
Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title_full Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title_fullStr Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title_full_unstemmed Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title_short Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking
title_sort highly stable and sensitive fluorescent probes (lysoprobes) for lysosomal labeling and tracking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341211/
https://www.ncbi.nlm.nih.gov/pubmed/25715948
http://dx.doi.org/10.1038/srep08576
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