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Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006
INTRODUCTION: Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection (ALRI) in young children. RSV strains have been divided into 2 major antigenic groups (A and B), which are further divided into several genotypes, but very little is known about its circulat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The African Field Epidemiology Network
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341265/ https://www.ncbi.nlm.nih.gov/pubmed/25745535 http://dx.doi.org/10.11604/pamj.2014.19.128.4749 |
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author | Obodai, Evangeline Asmah, Richard Boamah, Isaac Goka, Bamenla Odoom, John Kofi Adiku, Theophilus |
author_facet | Obodai, Evangeline Asmah, Richard Boamah, Isaac Goka, Bamenla Odoom, John Kofi Adiku, Theophilus |
author_sort | Obodai, Evangeline |
collection | PubMed |
description | INTRODUCTION: Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection (ALRI) in young children. RSV strains have been divided into 2 major antigenic groups (A and B), which are further divided into several genotypes, but very little is known about its circulating genotypes in Ghana. This study characterized RSV genotypes detected in children with ALRI in Accra between February and November 2006. METHODS: Nasopharyngeal aspirates (NPA) were obtained from children diagnosed with ALRI between February and November 2006. The NPA were screened for RSV using a nested multiplex reverse transcriptase polymerase chain reaction (RT-PCR) method for genotyping RSV. Viral RNA was extracted from the NPA using guanidinium isothiocyanate method and purified with an RNAID commercial kit. Care-givers gave their consent prior to specimen collection. Administered questionnaires captured information on patient demographic and clinical history. RESULTS: A total of 53 children were enrolled in the study with a male to female ratio of 3:1. Of the 53 NPA analyzed, 60.4% (32/53) were positive for RSV. Subsequent genotypic analysis showed that 72% (23/32) of the 60.4% RSV infections were RSV B only and 28% (9/32) were co-infections of both RSV A and B. Children between the ages of 2 - 12 months were the most affected age group per an RSV infection rate of 37.5% (12/32). No significant difference was detected in the recovery rate of ALRI (98.1%) and RSV (96.9%) positive patients from the infection. One patient died resulting in a mortality rate of 3.1%. Bronchopneumonia (20 out of 32 cases) was the major diagnosis on admission. RSV infection was seasonal dependent, described by 2 peaks in October and April-May. CONCLUSION: Both RSV A and RSV B genotypes co-circulated during the study period with RSV B predominating. RSV may possibly be the main pathogen of lower respiratory tract illness during epidemics in the wet seasons. Genotyping by the multiplex RT-PCR is one of the first attempts at molecular diagnosis of RSV infection in Ghana. |
format | Online Article Text |
id | pubmed-4341265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-43412652015-03-05 Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 Obodai, Evangeline Asmah, Richard Boamah, Isaac Goka, Bamenla Odoom, John Kofi Adiku, Theophilus Pan Afr Med J Research INTRODUCTION: Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection (ALRI) in young children. RSV strains have been divided into 2 major antigenic groups (A and B), which are further divided into several genotypes, but very little is known about its circulating genotypes in Ghana. This study characterized RSV genotypes detected in children with ALRI in Accra between February and November 2006. METHODS: Nasopharyngeal aspirates (NPA) were obtained from children diagnosed with ALRI between February and November 2006. The NPA were screened for RSV using a nested multiplex reverse transcriptase polymerase chain reaction (RT-PCR) method for genotyping RSV. Viral RNA was extracted from the NPA using guanidinium isothiocyanate method and purified with an RNAID commercial kit. Care-givers gave their consent prior to specimen collection. Administered questionnaires captured information on patient demographic and clinical history. RESULTS: A total of 53 children were enrolled in the study with a male to female ratio of 3:1. Of the 53 NPA analyzed, 60.4% (32/53) were positive for RSV. Subsequent genotypic analysis showed that 72% (23/32) of the 60.4% RSV infections were RSV B only and 28% (9/32) were co-infections of both RSV A and B. Children between the ages of 2 - 12 months were the most affected age group per an RSV infection rate of 37.5% (12/32). No significant difference was detected in the recovery rate of ALRI (98.1%) and RSV (96.9%) positive patients from the infection. One patient died resulting in a mortality rate of 3.1%. Bronchopneumonia (20 out of 32 cases) was the major diagnosis on admission. RSV infection was seasonal dependent, described by 2 peaks in October and April-May. CONCLUSION: Both RSV A and RSV B genotypes co-circulated during the study period with RSV B predominating. RSV may possibly be the main pathogen of lower respiratory tract illness during epidemics in the wet seasons. Genotyping by the multiplex RT-PCR is one of the first attempts at molecular diagnosis of RSV infection in Ghana. The African Field Epidemiology Network 2014-10-06 /pmc/articles/PMC4341265/ /pubmed/25745535 http://dx.doi.org/10.11604/pamj.2014.19.128.4749 Text en © John Kofi Odoom et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Obodai, Evangeline Asmah, Richard Boamah, Isaac Goka, Bamenla Odoom, John Kofi Adiku, Theophilus Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title | Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title_full | Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title_fullStr | Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title_full_unstemmed | Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title_short | Respiratory syncytial virus genotypes circulating in urban Ghana: february to november 2006 |
title_sort | respiratory syncytial virus genotypes circulating in urban ghana: february to november 2006 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341265/ https://www.ncbi.nlm.nih.gov/pubmed/25745535 http://dx.doi.org/10.11604/pamj.2014.19.128.4749 |
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