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The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342073/ https://www.ncbi.nlm.nih.gov/pubmed/25767554 http://dx.doi.org/10.1155/2015/596109 |
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author | Husin, Fitrien Chan, Yean Yean Gan, Siew Hua Sulaiman, Siti Amrah Shueb, Rafidah Hanim |
author_facet | Husin, Fitrien Chan, Yean Yean Gan, Siew Hua Sulaiman, Siti Amrah Shueb, Rafidah Hanim |
author_sort | Husin, Fitrien |
collection | PubMed |
description | Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. |
format | Online Article Text |
id | pubmed-4342073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43420732015-03-12 The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication Husin, Fitrien Chan, Yean Yean Gan, Siew Hua Sulaiman, Siti Amrah Shueb, Rafidah Hanim Evid Based Complement Alternat Med Research Article Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. Hindawi Publishing Corporation 2015 2015-02-12 /pmc/articles/PMC4342073/ /pubmed/25767554 http://dx.doi.org/10.1155/2015/596109 Text en Copyright © 2015 Fitrien Husin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Husin, Fitrien Chan, Yean Yean Gan, Siew Hua Sulaiman, Siti Amrah Shueb, Rafidah Hanim The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title_full | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title_fullStr | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title_full_unstemmed | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title_short | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication |
title_sort | effect of hydrocotyle sibthorpioides lam. extracts on in vitro dengue replication |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342073/ https://www.ncbi.nlm.nih.gov/pubmed/25767554 http://dx.doi.org/10.1155/2015/596109 |
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