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The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication

Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol...

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Autores principales: Husin, Fitrien, Chan, Yean Yean, Gan, Siew Hua, Sulaiman, Siti Amrah, Shueb, Rafidah Hanim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342073/
https://www.ncbi.nlm.nih.gov/pubmed/25767554
http://dx.doi.org/10.1155/2015/596109
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author Husin, Fitrien
Chan, Yean Yean
Gan, Siew Hua
Sulaiman, Siti Amrah
Shueb, Rafidah Hanim
author_facet Husin, Fitrien
Chan, Yean Yean
Gan, Siew Hua
Sulaiman, Siti Amrah
Shueb, Rafidah Hanim
author_sort Husin, Fitrien
collection PubMed
description Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus.
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spelling pubmed-43420732015-03-12 The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication Husin, Fitrien Chan, Yean Yean Gan, Siew Hua Sulaiman, Siti Amrah Shueb, Rafidah Hanim Evid Based Complement Alternat Med Research Article Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. Hindawi Publishing Corporation 2015 2015-02-12 /pmc/articles/PMC4342073/ /pubmed/25767554 http://dx.doi.org/10.1155/2015/596109 Text en Copyright © 2015 Fitrien Husin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Husin, Fitrien
Chan, Yean Yean
Gan, Siew Hua
Sulaiman, Siti Amrah
Shueb, Rafidah Hanim
The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title_full The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title_fullStr The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title_full_unstemmed The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title_short The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication
title_sort effect of hydrocotyle sibthorpioides lam. extracts on in vitro dengue replication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342073/
https://www.ncbi.nlm.nih.gov/pubmed/25767554
http://dx.doi.org/10.1155/2015/596109
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