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Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility

ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as...

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Autores principales: Song, Tingting, Zheng, Yi, Wang, Yarong, Katz, Zachary, Liu, Xin, Chen, Shaoying, Singer, Robert H., Gu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342582/
https://www.ncbi.nlm.nih.gov/pubmed/25588836
http://dx.doi.org/10.1242/jcs.161679
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author Song, Tingting
Zheng, Yi
Wang, Yarong
Katz, Zachary
Liu, Xin
Chen, Shaoying
Singer, Robert H.
Gu, Wei
author_facet Song, Tingting
Zheng, Yi
Wang, Yarong
Katz, Zachary
Liu, Xin
Chen, Shaoying
Singer, Robert H.
Gu, Wei
author_sort Song, Tingting
collection PubMed
description ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of β-actin messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes with the β-actin mRNA, and the ability of KIF11 to transport β-actin mRNA is dependent on ZBP1. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes β-actin mRNA and affects cell migration. Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein.
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spelling pubmed-43425822015-03-10 Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility Song, Tingting Zheng, Yi Wang, Yarong Katz, Zachary Liu, Xin Chen, Shaoying Singer, Robert H. Gu, Wei J Cell Sci Research Article ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of β-actin messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes with the β-actin mRNA, and the ability of KIF11 to transport β-actin mRNA is dependent on ZBP1. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes β-actin mRNA and affects cell migration. Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein. The Company of Biologists 2015-03-01 /pmc/articles/PMC4342582/ /pubmed/25588836 http://dx.doi.org/10.1242/jcs.161679 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Song, Tingting
Zheng, Yi
Wang, Yarong
Katz, Zachary
Liu, Xin
Chen, Shaoying
Singer, Robert H.
Gu, Wei
Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title_full Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title_fullStr Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title_full_unstemmed Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title_short Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
title_sort specific interaction of kif11 with zbp1 regulates the transport of β-actin mrna and cell motility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342582/
https://www.ncbi.nlm.nih.gov/pubmed/25588836
http://dx.doi.org/10.1242/jcs.161679
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