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Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility
ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342582/ https://www.ncbi.nlm.nih.gov/pubmed/25588836 http://dx.doi.org/10.1242/jcs.161679 |
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author | Song, Tingting Zheng, Yi Wang, Yarong Katz, Zachary Liu, Xin Chen, Shaoying Singer, Robert H. Gu, Wei |
author_facet | Song, Tingting Zheng, Yi Wang, Yarong Katz, Zachary Liu, Xin Chen, Shaoying Singer, Robert H. Gu, Wei |
author_sort | Song, Tingting |
collection | PubMed |
description | ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of β-actin messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes with the β-actin mRNA, and the ability of KIF11 to transport β-actin mRNA is dependent on ZBP1. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes β-actin mRNA and affects cell migration. Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein. |
format | Online Article Text |
id | pubmed-4342582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-43425822015-03-10 Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility Song, Tingting Zheng, Yi Wang, Yarong Katz, Zachary Liu, Xin Chen, Shaoying Singer, Robert H. Gu, Wei J Cell Sci Research Article ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of β-actin messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes with the β-actin mRNA, and the ability of KIF11 to transport β-actin mRNA is dependent on ZBP1. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes β-actin mRNA and affects cell migration. Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein. The Company of Biologists 2015-03-01 /pmc/articles/PMC4342582/ /pubmed/25588836 http://dx.doi.org/10.1242/jcs.161679 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Song, Tingting Zheng, Yi Wang, Yarong Katz, Zachary Liu, Xin Chen, Shaoying Singer, Robert H. Gu, Wei Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title | Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title_full | Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title_fullStr | Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title_full_unstemmed | Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title_short | Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility |
title_sort | specific interaction of kif11 with zbp1 regulates the transport of β-actin mrna and cell motility |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342582/ https://www.ncbi.nlm.nih.gov/pubmed/25588836 http://dx.doi.org/10.1242/jcs.161679 |
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