Cargando…
A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form
BACKGROUND: Mild solubilization of inclusion bodies has attracted attention in recent days, with an objective to preserve the existing native-like secondary structure of proteins, reduce protein aggregation during refolding and recovering high amount of bioactive proteins from inclusion bodies. RESU...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2015
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4343044/ https://www.ncbi.nlm.nih.gov/pubmed/25879903 http://dx.doi.org/10.1186/s12934-015-0208-6 |