Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). This strategy has been used to construct both intermolecular and intramolecular ratiom...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344385/ https://www.ncbi.nlm.nih.gov/pubmed/25622108 http://dx.doi.org/10.1038/nmeth.3261 |
Sumario: | We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). This strategy has been used to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics, and other second messenger signaling activities. |
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