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Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). This strategy has been used to construct both intermolecular and intramolecular ratiom...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344385/ https://www.ncbi.nlm.nih.gov/pubmed/25622108 http://dx.doi.org/10.1038/nmeth.3261 |
| _version_ | 1782359415291117568 |
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| author | Ding, Yidan Li, Jiao Enterina, Jhon Ralph Shen, Yi Zhang, Issan Tewson, Paul H. Mo, Gary C. H. Zhang, Jin Quinn, Anne Marie Hughes, Thomas E. Maysinger, Dusica Alford, Spencer C. Zhang, Yan Campbell, Robert E. |
| author_facet | Ding, Yidan Li, Jiao Enterina, Jhon Ralph Shen, Yi Zhang, Issan Tewson, Paul H. Mo, Gary C. H. Zhang, Jin Quinn, Anne Marie Hughes, Thomas E. Maysinger, Dusica Alford, Spencer C. Zhang, Yan Campbell, Robert E. |
| author_sort | Ding, Yidan |
| collection | PubMed |
| description | We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). This strategy has been used to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics, and other second messenger signaling activities. |
| format | Online Article Text |
| id | pubmed-4344385 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-43443852015-09-01 Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange Ding, Yidan Li, Jiao Enterina, Jhon Ralph Shen, Yi Zhang, Issan Tewson, Paul H. Mo, Gary C. H. Zhang, Jin Quinn, Anne Marie Hughes, Thomas E. Maysinger, Dusica Alford, Spencer C. Zhang, Yan Campbell, Robert E. Nat Methods Article We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). This strategy has been used to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics, and other second messenger signaling activities. 2015-01-26 2015-03 /pmc/articles/PMC4344385/ /pubmed/25622108 http://dx.doi.org/10.1038/nmeth.3261 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Ding, Yidan Li, Jiao Enterina, Jhon Ralph Shen, Yi Zhang, Issan Tewson, Paul H. Mo, Gary C. H. Zhang, Jin Quinn, Anne Marie Hughes, Thomas E. Maysinger, Dusica Alford, Spencer C. Zhang, Yan Campbell, Robert E. Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title | Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title_full | Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title_fullStr | Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title_full_unstemmed | Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title_short | Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| title_sort | ratiometric biosensors based on dimerization-dependent fluorescent protein exchange |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344385/ https://www.ncbi.nlm.nih.gov/pubmed/25622108 http://dx.doi.org/10.1038/nmeth.3261 |
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