Improved and expanded Q-system reagents for genetic manipulations

The Q-system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here new variants of the Q-system transcriptional activator, including QF2, that allows the Q-system to drive strong and ubiqui...

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Detalles Bibliográficos
Autores principales: Riabinina, Olena, Luginbuhl, David, Marr, Elizabeth, Liu, Sha, Wu, Mark N., Luo, Liqun, Potter, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344399/
https://www.ncbi.nlm.nih.gov/pubmed/25581800
http://dx.doi.org/10.1038/nmeth.3250
Descripción
Sumario:The Q-system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here new variants of the Q-system transcriptional activator, including QF2, that allows the Q-system to drive strong and ubiquitous expression for the first time in all tissues. Our new QF2, GAL4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila.

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