Improved and expanded Q-system reagents for genetic manipulations

The Q-system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here new variants of the Q-system transcriptional activator, including QF2, that allows the Q-system to drive strong and ubiqui...

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Detalles Bibliográficos
Autores principales: Riabinina, Olena, Luginbuhl, David, Marr, Elizabeth, Liu, Sha, Wu, Mark N., Luo, Liqun, Potter, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344399/
https://www.ncbi.nlm.nih.gov/pubmed/25581800
http://dx.doi.org/10.1038/nmeth.3250
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author Riabinina, Olena
Luginbuhl, David
Marr, Elizabeth
Liu, Sha
Wu, Mark N.
Luo, Liqun
Potter, Christopher J.
author_facet Riabinina, Olena
Luginbuhl, David
Marr, Elizabeth
Liu, Sha
Wu, Mark N.
Luo, Liqun
Potter, Christopher J.
author_sort Riabinina, Olena
collection PubMed
description The Q-system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here new variants of the Q-system transcriptional activator, including QF2, that allows the Q-system to drive strong and ubiquitous expression for the first time in all tissues. Our new QF2, GAL4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila.
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spelling pubmed-43443992015-09-01 Improved and expanded Q-system reagents for genetic manipulations Riabinina, Olena Luginbuhl, David Marr, Elizabeth Liu, Sha Wu, Mark N. Luo, Liqun Potter, Christopher J. Nat Methods Article The Q-system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here new variants of the Q-system transcriptional activator, including QF2, that allows the Q-system to drive strong and ubiquitous expression for the first time in all tissues. Our new QF2, GAL4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila. 2015-01-12 2015-03 /pmc/articles/PMC4344399/ /pubmed/25581800 http://dx.doi.org/10.1038/nmeth.3250 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Riabinina, Olena
Luginbuhl, David
Marr, Elizabeth
Liu, Sha
Wu, Mark N.
Luo, Liqun
Potter, Christopher J.
Improved and expanded Q-system reagents for genetic manipulations
title Improved and expanded Q-system reagents for genetic manipulations
title_full Improved and expanded Q-system reagents for genetic manipulations
title_fullStr Improved and expanded Q-system reagents for genetic manipulations
title_full_unstemmed Improved and expanded Q-system reagents for genetic manipulations
title_short Improved and expanded Q-system reagents for genetic manipulations
title_sort improved and expanded q-system reagents for genetic manipulations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344399/
https://www.ncbi.nlm.nih.gov/pubmed/25581800
http://dx.doi.org/10.1038/nmeth.3250
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