Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluore...

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Autores principales: Paez Segala, Maria G., Sun, Mei G., Shtengel, Gleb, Viswanathan, Sarada, Baird, Michelle A., Macklin, John J., Patel, Ronak, Allen, John R., Howe, Elizabeth S., Piszczek, Grzegorz, Hess, Harald F., Davidson, Michael W., Wang, Yalin, Looger, Loren L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344411/
https://www.ncbi.nlm.nih.gov/pubmed/25581799
http://dx.doi.org/10.1038/nmeth.3225
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author Paez Segala, Maria G.
Sun, Mei G.
Shtengel, Gleb
Viswanathan, Sarada
Baird, Michelle A.
Macklin, John J.
Patel, Ronak
Allen, John R.
Howe, Elizabeth S.
Piszczek, Grzegorz
Hess, Harald F.
Davidson, Michael W.
Wang, Yalin
Looger, Loren L.
author_facet Paez Segala, Maria G.
Sun, Mei G.
Shtengel, Gleb
Viswanathan, Sarada
Baird, Michelle A.
Macklin, John J.
Patel, Ronak
Allen, John R.
Howe, Elizabeth S.
Piszczek, Grzegorz
Hess, Harald F.
Davidson, Michael W.
Wang, Yalin
Looger, Loren L.
author_sort Paez Segala, Maria G.
collection PubMed
description Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluorescent protein that function normally in heavily fixed (0.5–1% OsO(4)), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.
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spelling pubmed-43444112015-09-01 Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy Paez Segala, Maria G. Sun, Mei G. Shtengel, Gleb Viswanathan, Sarada Baird, Michelle A. Macklin, John J. Patel, Ronak Allen, John R. Howe, Elizabeth S. Piszczek, Grzegorz Hess, Harald F. Davidson, Michael W. Wang, Yalin Looger, Loren L. Nat Methods Article Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluorescent protein that function normally in heavily fixed (0.5–1% OsO(4)), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy. 2015-01-12 2015-03 /pmc/articles/PMC4344411/ /pubmed/25581799 http://dx.doi.org/10.1038/nmeth.3225 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Paez Segala, Maria G.
Sun, Mei G.
Shtengel, Gleb
Viswanathan, Sarada
Baird, Michelle A.
Macklin, John J.
Patel, Ronak
Allen, John R.
Howe, Elizabeth S.
Piszczek, Grzegorz
Hess, Harald F.
Davidson, Michael W.
Wang, Yalin
Looger, Loren L.
Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title_full Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title_fullStr Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title_full_unstemmed Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title_short Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
title_sort fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344411/
https://www.ncbi.nlm.nih.gov/pubmed/25581799
http://dx.doi.org/10.1038/nmeth.3225
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