Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluore...

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Detalles Bibliográficos
Autores principales: Paez Segala, Maria G., Sun, Mei G., Shtengel, Gleb, Viswanathan, Sarada, Baird, Michelle A., Macklin, John J., Patel, Ronak, Allen, John R., Howe, Elizabeth S., Piszczek, Grzegorz, Hess, Harald F., Davidson, Michael W., Wang, Yalin, Looger, Loren L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344411/
https://www.ncbi.nlm.nih.gov/pubmed/25581799
http://dx.doi.org/10.1038/nmeth.3225

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