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Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum

Microencapsulation using extrusion and emulsion techniques was prepared for Bifidobacterium longum protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. Eleutherine americana was used as the co-encapsulating agent. Hydrolysis of...

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Detalles Bibliográficos
Autores principales: Phoem, Atchara N, Chanthachum, Suphitchaya, Voravuthikunchai, Supayang P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344563/
https://www.ncbi.nlm.nih.gov/pubmed/25629556
http://dx.doi.org/10.3390/nu7020831
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author Phoem, Atchara N
Chanthachum, Suphitchaya
Voravuthikunchai, Supayang P
author_facet Phoem, Atchara N
Chanthachum, Suphitchaya
Voravuthikunchai, Supayang P
author_sort Phoem, Atchara N
collection PubMed
description Microencapsulation using extrusion and emulsion techniques was prepared for Bifidobacterium longum protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. Eleutherine americana was used as the co-encapsulating agent. Hydrolysis of E. americana by gastric and intestinal juices was also determined. E. americana and its oligosaccharide extract demonstrated their resistance to low pH and partial tolerance to human α-amylase. Microencapsulated B. longum with E. americana and oligosaccharide extract prepared by the extrusion technique survived better than that by the emulsion technique under adverse conditions. Survival of microencapsulated cells after exposure to the juices and refrigeration storage was higher than free cells at Weeks 2 and 4. In addition, the viability of microencapsulated cells was better than free cells at 65 °C for 15 min. This work suggested that microencapsulated B. longum with E. americana offers the effective delivery of probiotics to colon and maintains their survival in food products.
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spelling pubmed-43445632015-03-18 Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum Phoem, Atchara N Chanthachum, Suphitchaya Voravuthikunchai, Supayang P Nutrients Article Microencapsulation using extrusion and emulsion techniques was prepared for Bifidobacterium longum protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. Eleutherine americana was used as the co-encapsulating agent. Hydrolysis of E. americana by gastric and intestinal juices was also determined. E. americana and its oligosaccharide extract demonstrated their resistance to low pH and partial tolerance to human α-amylase. Microencapsulated B. longum with E. americana and oligosaccharide extract prepared by the extrusion technique survived better than that by the emulsion technique under adverse conditions. Survival of microencapsulated cells after exposure to the juices and refrigeration storage was higher than free cells at Weeks 2 and 4. In addition, the viability of microencapsulated cells was better than free cells at 65 °C for 15 min. This work suggested that microencapsulated B. longum with E. americana offers the effective delivery of probiotics to colon and maintains their survival in food products. MDPI 2015-01-26 /pmc/articles/PMC4344563/ /pubmed/25629556 http://dx.doi.org/10.3390/nu7020831 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Phoem, Atchara N
Chanthachum, Suphitchaya
Voravuthikunchai, Supayang P
Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title_full Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title_fullStr Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title_full_unstemmed Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title_short Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum
title_sort preparation of eleutherine americana-alginate complex microcapsules and application in bifidobacterium longum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344563/
https://www.ncbi.nlm.nih.gov/pubmed/25629556
http://dx.doi.org/10.3390/nu7020831
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