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Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes

Nutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitr...

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Autores principales: Romacho, Tania, Glosse, Philipp, Richter, Isabel, Elsen, Manuela, Schoemaker, Marieke H., van Tol, Eric A., Eckel, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344565/
https://www.ncbi.nlm.nih.gov/pubmed/25629558
http://dx.doi.org/10.3390/nu7020865
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author Romacho, Tania
Glosse, Philipp
Richter, Isabel
Elsen, Manuela
Schoemaker, Marieke H.
van Tol, Eric A.
Eckel, Jürgen
author_facet Romacho, Tania
Glosse, Philipp
Richter, Isabel
Elsen, Manuela
Schoemaker, Marieke H.
van Tol, Eric A.
Eckel, Jürgen
author_sort Romacho, Tania
collection PubMed
description Nutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitro. Incubation conditions with the different nutritional factors were validated by assessing cell vitality with lactate dehydrogenase (LDH) release and neutral red incorporation. Intracellular triglyceride content was assessed with Oil Red O staining. The effect of eCH, a non-peptidic amino acid mixture (AA), and long-chain polyunsaturated fatty acids (LC-PUFAs) on adiponectin and leptin secretion was determined by enzyme-linked immunosorbent assay (ELISA). Intracellular adiponectin expression and nuclear factor-κB (NF-κB) activation were analyzed by Western blot, while monocyte chemoattractant protein-1 (MCP-1) release was explored by ELISA. The eCH concentration dependently increased adiponectin secretion in human primary adipocytes through its intrinsic peptide bioactivity, since the non-peptidic mixture, AA, could not mimic eCH’s effects on adiponectin secretion. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and DHA combined with arachidonic acid (ARA) upregulated adiponectin secretion. However, only DHA and DHA/ARA exerted a potentanti-inflammatory effect reflected by prevention of tumor necrosis factor-α (TNF-α) induced NF-κB activation and MCP-1 secretion in human adipocytes. eCH and DHA alone or in combination with ARA, may hold the key for nutritional programming through their anti-inflammatory action to prevent diseases with low-grade chronic inflammation such as obesity or diabetes.
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spelling pubmed-43445652015-03-18 Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes Romacho, Tania Glosse, Philipp Richter, Isabel Elsen, Manuela Schoemaker, Marieke H. van Tol, Eric A. Eckel, Jürgen Nutrients Article Nutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitro. Incubation conditions with the different nutritional factors were validated by assessing cell vitality with lactate dehydrogenase (LDH) release and neutral red incorporation. Intracellular triglyceride content was assessed with Oil Red O staining. The effect of eCH, a non-peptidic amino acid mixture (AA), and long-chain polyunsaturated fatty acids (LC-PUFAs) on adiponectin and leptin secretion was determined by enzyme-linked immunosorbent assay (ELISA). Intracellular adiponectin expression and nuclear factor-κB (NF-κB) activation were analyzed by Western blot, while monocyte chemoattractant protein-1 (MCP-1) release was explored by ELISA. The eCH concentration dependently increased adiponectin secretion in human primary adipocytes through its intrinsic peptide bioactivity, since the non-peptidic mixture, AA, could not mimic eCH’s effects on adiponectin secretion. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and DHA combined with arachidonic acid (ARA) upregulated adiponectin secretion. However, only DHA and DHA/ARA exerted a potentanti-inflammatory effect reflected by prevention of tumor necrosis factor-α (TNF-α) induced NF-κB activation and MCP-1 secretion in human adipocytes. eCH and DHA alone or in combination with ARA, may hold the key for nutritional programming through their anti-inflammatory action to prevent diseases with low-grade chronic inflammation such as obesity or diabetes. MDPI 2015-01-26 /pmc/articles/PMC4344565/ /pubmed/25629558 http://dx.doi.org/10.3390/nu7020865 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Romacho, Tania
Glosse, Philipp
Richter, Isabel
Elsen, Manuela
Schoemaker, Marieke H.
van Tol, Eric A.
Eckel, Jürgen
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title_full Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title_fullStr Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title_full_unstemmed Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title_short Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
title_sort nutritional ingredients modulate adipokine secretion and inflammation in human primary adipocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344565/
https://www.ncbi.nlm.nih.gov/pubmed/25629558
http://dx.doi.org/10.3390/nu7020865
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