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Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight
Fusarium graminearum is responsible for Fusarium head blight (FHB), which is a destructive disease of wheat that makes its quality unsuitable for end use. To understand the temporal molecular response against this pathogen, microarray gene expression analysis was carried out at two time points on th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344645/ https://www.ncbi.nlm.nih.gov/pubmed/25690694 http://dx.doi.org/10.3390/toxins7020604 |
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author | Kosaka, Ayumi Manickavelu, Alagu Kajihara, Daniela Nakagawa, Hiroyuki Ban, Tomohiro |
author_facet | Kosaka, Ayumi Manickavelu, Alagu Kajihara, Daniela Nakagawa, Hiroyuki Ban, Tomohiro |
author_sort | Kosaka, Ayumi |
collection | PubMed |
description | Fusarium graminearum is responsible for Fusarium head blight (FHB), which is a destructive disease of wheat that makes its quality unsuitable for end use. To understand the temporal molecular response against this pathogen, microarray gene expression analysis was carried out at two time points on three wheat genotypes, the spikes of which were infected by Fusarium graminearum. The greatest number of genes was upregulated in Nobeokabouzu-komugi followed by Sumai 3, whereas the minimum expression in Gamenya was at three days after inoculation (dai). In Nobeokabouzu-komugi, high expression of detoxification genes, such as multidrug-resistant protein, multidrug resistance-associated protein, UDP-glycosyltransferase and ABC transporters, in addition to systemic defense-related genes, were identified at the early stage of infection. This early response of the highly-resistant genotype implies a different resistance response from the other resistant genotype, Sumai 3, primarily containing local defense-related genes, such as cell wall defense genes. In Gamenya, the expression of all three functional groups was minimal. The differences in these molecular responses with respect to the time points confirmed the variation in the genotypes. For the first time, we report the nature of gene expression in the FHB-highly resistant cv. Nobeokabouzu-komugi during the disease establishment stage and the possible underlying molecular response. |
format | Online Article Text |
id | pubmed-4344645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43446452015-03-18 Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight Kosaka, Ayumi Manickavelu, Alagu Kajihara, Daniela Nakagawa, Hiroyuki Ban, Tomohiro Toxins (Basel) Article Fusarium graminearum is responsible for Fusarium head blight (FHB), which is a destructive disease of wheat that makes its quality unsuitable for end use. To understand the temporal molecular response against this pathogen, microarray gene expression analysis was carried out at two time points on three wheat genotypes, the spikes of which were infected by Fusarium graminearum. The greatest number of genes was upregulated in Nobeokabouzu-komugi followed by Sumai 3, whereas the minimum expression in Gamenya was at three days after inoculation (dai). In Nobeokabouzu-komugi, high expression of detoxification genes, such as multidrug-resistant protein, multidrug resistance-associated protein, UDP-glycosyltransferase and ABC transporters, in addition to systemic defense-related genes, were identified at the early stage of infection. This early response of the highly-resistant genotype implies a different resistance response from the other resistant genotype, Sumai 3, primarily containing local defense-related genes, such as cell wall defense genes. In Gamenya, the expression of all three functional groups was minimal. The differences in these molecular responses with respect to the time points confirmed the variation in the genotypes. For the first time, we report the nature of gene expression in the FHB-highly resistant cv. Nobeokabouzu-komugi during the disease establishment stage and the possible underlying molecular response. MDPI 2015-02-16 /pmc/articles/PMC4344645/ /pubmed/25690694 http://dx.doi.org/10.3390/toxins7020604 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kosaka, Ayumi Manickavelu, Alagu Kajihara, Daniela Nakagawa, Hiroyuki Ban, Tomohiro Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title | Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title_full | Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title_fullStr | Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title_full_unstemmed | Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title_short | Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight |
title_sort | altered gene expression profiles of wheat genotypes against fusarium head blight |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344645/ https://www.ncbi.nlm.nih.gov/pubmed/25690694 http://dx.doi.org/10.3390/toxins7020604 |
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