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Surveillance of Hepatitis E Virus Contamination in Shellfish in China
Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contami...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344708/ https://www.ncbi.nlm.nih.gov/pubmed/25689991 http://dx.doi.org/10.3390/ijerph120202026 |
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author | Gao, Shenyang Li, Dandan Zha, Enhui Zhou, Tiezhong Wang, Shen Yue, Xiqing |
author_facet | Gao, Shenyang Li, Dandan Zha, Enhui Zhou, Tiezhong Wang, Shen Yue, Xiqing |
author_sort | Gao, Shenyang |
collection | PubMed |
description | Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks. Methods: samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis. Results: the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish. HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group. Conclusions: 13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish. Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas. |
format | Online Article Text |
id | pubmed-4344708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43447082015-03-18 Surveillance of Hepatitis E Virus Contamination in Shellfish in China Gao, Shenyang Li, Dandan Zha, Enhui Zhou, Tiezhong Wang, Shen Yue, Xiqing Int J Environ Res Public Health Short Communication Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks. Methods: samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis. Results: the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish. HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group. Conclusions: 13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish. Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas. MDPI 2015-02-11 2015-02 /pmc/articles/PMC4344708/ /pubmed/25689991 http://dx.doi.org/10.3390/ijerph120202026 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Short Communication Gao, Shenyang Li, Dandan Zha, Enhui Zhou, Tiezhong Wang, Shen Yue, Xiqing Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title | Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title_full | Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title_fullStr | Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title_full_unstemmed | Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title_short | Surveillance of Hepatitis E Virus Contamination in Shellfish in China |
title_sort | surveillance of hepatitis e virus contamination in shellfish in china |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344708/ https://www.ncbi.nlm.nih.gov/pubmed/25689991 http://dx.doi.org/10.3390/ijerph120202026 |
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