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Surveillance of Hepatitis E Virus Contamination in Shellfish in China

Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contami...

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Autores principales: Gao, Shenyang, Li, Dandan, Zha, Enhui, Zhou, Tiezhong, Wang, Shen, Yue, Xiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344708/
https://www.ncbi.nlm.nih.gov/pubmed/25689991
http://dx.doi.org/10.3390/ijerph120202026
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author Gao, Shenyang
Li, Dandan
Zha, Enhui
Zhou, Tiezhong
Wang, Shen
Yue, Xiqing
author_facet Gao, Shenyang
Li, Dandan
Zha, Enhui
Zhou, Tiezhong
Wang, Shen
Yue, Xiqing
author_sort Gao, Shenyang
collection PubMed
description Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks. Methods: samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis. Results: the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish.  HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group. Conclusions: 13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish.  Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas.
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spelling pubmed-43447082015-03-18 Surveillance of Hepatitis E Virus Contamination in Shellfish in China Gao, Shenyang Li, Dandan Zha, Enhui Zhou, Tiezhong Wang, Shen Yue, Xiqing Int J Environ Res Public Health Short Communication Background: Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks. Methods: samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis. Results: the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish.  HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group. Conclusions: 13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish.  Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas. MDPI 2015-02-11 2015-02 /pmc/articles/PMC4344708/ /pubmed/25689991 http://dx.doi.org/10.3390/ijerph120202026 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Gao, Shenyang
Li, Dandan
Zha, Enhui
Zhou, Tiezhong
Wang, Shen
Yue, Xiqing
Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title_full Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title_fullStr Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title_full_unstemmed Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title_short Surveillance of Hepatitis E Virus Contamination in Shellfish in China
title_sort surveillance of hepatitis e virus contamination in shellfish in china
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344708/
https://www.ncbi.nlm.nih.gov/pubmed/25689991
http://dx.doi.org/10.3390/ijerph120202026
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