Cargando…

Reporters for sensitive and quantitative measurement of auxin response

Visualization of hormonal signaling input and output is of key importance for understanding regulation of multicellular development. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack sensitivity or precision to visualize these. We developed a...

Descripción completa

Detalles Bibliográficos
Autores principales: Liao, Che-Yang, Smet, Wouter, Brunoud, Geraldine, Yoshida, Saiko, Vernoux, Teva, Weijers, Dolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344836/
https://www.ncbi.nlm.nih.gov/pubmed/25643149
http://dx.doi.org/10.1038/nmeth.3279
_version_ 1782359495802880000
author Liao, Che-Yang
Smet, Wouter
Brunoud, Geraldine
Yoshida, Saiko
Vernoux, Teva
Weijers, Dolf
author_facet Liao, Che-Yang
Smet, Wouter
Brunoud, Geraldine
Yoshida, Saiko
Vernoux, Teva
Weijers, Dolf
author_sort Liao, Che-Yang
collection PubMed
description Visualization of hormonal signaling input and output is of key importance for understanding regulation of multicellular development. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack sensitivity or precision to visualize these. We developed a set of novel fluorescent reporters that allow sensitive and semi-quantitative readout of auxin responses at cellular resolution in Arabidopsis. These generic tools are suitable for any transformable plant species.
format Online
Article
Text
id pubmed-4344836
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-43448362015-09-01 Reporters for sensitive and quantitative measurement of auxin response Liao, Che-Yang Smet, Wouter Brunoud, Geraldine Yoshida, Saiko Vernoux, Teva Weijers, Dolf Nat Methods Article Visualization of hormonal signaling input and output is of key importance for understanding regulation of multicellular development. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack sensitivity or precision to visualize these. We developed a set of novel fluorescent reporters that allow sensitive and semi-quantitative readout of auxin responses at cellular resolution in Arabidopsis. These generic tools are suitable for any transformable plant species. 2015-02-02 2015-03 /pmc/articles/PMC4344836/ /pubmed/25643149 http://dx.doi.org/10.1038/nmeth.3279 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Liao, Che-Yang
Smet, Wouter
Brunoud, Geraldine
Yoshida, Saiko
Vernoux, Teva
Weijers, Dolf
Reporters for sensitive and quantitative measurement of auxin response
title Reporters for sensitive and quantitative measurement of auxin response
title_full Reporters for sensitive and quantitative measurement of auxin response
title_fullStr Reporters for sensitive and quantitative measurement of auxin response
title_full_unstemmed Reporters for sensitive and quantitative measurement of auxin response
title_short Reporters for sensitive and quantitative measurement of auxin response
title_sort reporters for sensitive and quantitative measurement of auxin response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344836/
https://www.ncbi.nlm.nih.gov/pubmed/25643149
http://dx.doi.org/10.1038/nmeth.3279
work_keys_str_mv AT liaocheyang reportersforsensitiveandquantitativemeasurementofauxinresponse
AT smetwouter reportersforsensitiveandquantitativemeasurementofauxinresponse
AT brunoudgeraldine reportersforsensitiveandquantitativemeasurementofauxinresponse
AT yoshidasaiko reportersforsensitiveandquantitativemeasurementofauxinresponse
AT vernouxteva reportersforsensitiveandquantitativemeasurementofauxinresponse
AT weijersdolf reportersforsensitiveandquantitativemeasurementofauxinresponse