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The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules

BACKGROUND: Interactions of peripheral microtubule tips with the cell cortex are of crucial importance for nuclear migration, spindle orientation, centrosome positioning and directional cell movement. Microtubule plus end binding proteins are thought to mediate interactions of microtubule tips with...

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Autores principales: Hestermann, Andrea, Gräf, Ralph
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC434496/
https://www.ncbi.nlm.nih.gov/pubmed/15186508
http://dx.doi.org/10.1186/1471-2121-5-24
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author Hestermann, Andrea
Gräf, Ralph
author_facet Hestermann, Andrea
Gräf, Ralph
author_sort Hestermann, Andrea
collection PubMed
description BACKGROUND: Interactions of peripheral microtubule tips with the cell cortex are of crucial importance for nuclear migration, spindle orientation, centrosome positioning and directional cell movement. Microtubule plus end binding proteins are thought to mediate interactions of microtubule tips with cortical actin and membrane proteins in a dynein-dependent manner. XMAP215-family proteins are main regulators of microtubule plus end dynamics but so far they have not been implicated in the interactions of microtubule tips with the cell cortex. RESULTS: Here we show that overexpression of an N-terminal fragment of DdCP224, the Dictyostelium XMAP215 homologue, caused a collapse of the radial microtubule cytoskeleton, whereby microtubules lost contact with the cell cortex and were dragged behind like a comet tail of an unusually motile centrosome. This phenotype was indistinguishable from mutants overexpressing fragments of the dynein heavy chain or intermediate chain. Moreover, it was accompanied by dispersal of the Golgi apparatus and reduced cortical localization of the dynein heavy chain indicating a disrupted dynein/dynactin interaction. The interference of DdCP224 with cortical dynein function is strongly supported by the observations that DdCP224 and its N-terminal fragment colocalize with dynein and coimmunoprecipitate with dynein and dynactin. CONCLUSIONS: Our data show that XMAP215-like proteins are required for the interaction of microtubule plus ends with the cell cortex in interphase cells and strongly suggest that this function is mediated by dynein.
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spelling pubmed-4344962004-06-25 The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules Hestermann, Andrea Gräf, Ralph BMC Cell Biol Research Article BACKGROUND: Interactions of peripheral microtubule tips with the cell cortex are of crucial importance for nuclear migration, spindle orientation, centrosome positioning and directional cell movement. Microtubule plus end binding proteins are thought to mediate interactions of microtubule tips with cortical actin and membrane proteins in a dynein-dependent manner. XMAP215-family proteins are main regulators of microtubule plus end dynamics but so far they have not been implicated in the interactions of microtubule tips with the cell cortex. RESULTS: Here we show that overexpression of an N-terminal fragment of DdCP224, the Dictyostelium XMAP215 homologue, caused a collapse of the radial microtubule cytoskeleton, whereby microtubules lost contact with the cell cortex and were dragged behind like a comet tail of an unusually motile centrosome. This phenotype was indistinguishable from mutants overexpressing fragments of the dynein heavy chain or intermediate chain. Moreover, it was accompanied by dispersal of the Golgi apparatus and reduced cortical localization of the dynein heavy chain indicating a disrupted dynein/dynactin interaction. The interference of DdCP224 with cortical dynein function is strongly supported by the observations that DdCP224 and its N-terminal fragment colocalize with dynein and coimmunoprecipitate with dynein and dynactin. CONCLUSIONS: Our data show that XMAP215-like proteins are required for the interaction of microtubule plus ends with the cell cortex in interphase cells and strongly suggest that this function is mediated by dynein. BioMed Central 2004-06-08 /pmc/articles/PMC434496/ /pubmed/15186508 http://dx.doi.org/10.1186/1471-2121-5-24 Text en Copyright © 2004 Hestermann and Gräf; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Hestermann, Andrea
Gräf, Ralph
The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title_full The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title_fullStr The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title_full_unstemmed The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title_short The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules
title_sort xmap215-family protein ddcp224 is required for cortical interactions of microtubules
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC434496/
https://www.ncbi.nlm.nih.gov/pubmed/15186508
http://dx.doi.org/10.1186/1471-2121-5-24
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